TM-AFM threshold analysis of macromolecular orientation: A study of the orientation of IgG and IgE on mica surfaces

被引：45

作者：

Bergkvist, M

Carlsson, J

Karlsson, T

Oscarsson, S

机构：

[1] Malardalen Univ, Dept Chem Engn, S-63105 Eskilstuna, Sweden

[2] Pharmacia & Upjohn Diagnost AB, S-75182 Uppsala, Sweden

[3] Univ Uppsala, Surface Biotechnol Ctr, S-75123 Uppsala, Sweden

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 1998年 / 206卷 / 02期

关键词：

protein adsorption; orientation; TM-AFM; surface interaction; surface derivatization;

D O I：

10.1006/jcis.1998.5630

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Adsorption and orientation properties of two different types of immunoglobulin molecules on derivatized and native mica surfaces were investigated using TM-AFM. The analyses included height measurements at two different pH values and a new technique, presented here as threshold analysis, which displays the outer mantle shape of an adsorbed protein. A major difference in preferential orientation is observed upon comparing the adsorption of the two proteins onto the different surfaces. The characteristics of both the adsorbed immunoglobulin and the surface are important for any preferential orientation of the adsorbed protein. (C) 1998 Academic Press.

引用

页码：475 / 481

页数：7