Production of biological active murine IFN-γ by recombinant Lactococcus lactis

被引：25

作者：

Bermudez-Humaran, Luis G. ^{[1
]}

Cortes-Perez, Naima G. ^{[1
]}

L'Haridon, Rene ^{[2
]}

Langella, Philippe ^{[1
]}

机构：

[1] INRA, Unite Ecol & Physiol Syst Digestif, F-78352 Jouy En Josas, France

[2] INRA, Unite Virol & Immunol Mol, F-78352 Jouy En Josas, France

来源：

FEMS MICROBIOLOGY LETTERS | 2008年 / 280卷 / 02期

关键词：

Lactococcus lactis; IFN-gamma; cytokines; lactic acid bacteria; live vector;

D O I：

10.1111/j.1574-6968.2007.01038.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

IFN-gamma is a cytokine produced primarily by both T lymphocytes and natural killer cells and it is considered to be an attractive therapeutic molecule. In the present study, a DNA sequence encoding the mature murine IFN-gamma (muIFN-gamma) protein was cloned and expressed in the food-grade lactic acid bacterium Lactococcus lactis. The activity of recombinant muIFN-gamma produced by genetically engineered L. lactis was confirmed in an antiviral assay using MoV cells infected with Vesicular Stomatitis Virus. The data provide the first demonstration that a Gram-positive bacterium, L. lactis, is able to produce functional muIFN-gamma. This recombinant strain could lead to the development of a new, well-tolerated vector to deliver active muIFN-gamma at the mucosal level.

引用

页码：144 / 149

页数：6

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