Apoptosis induction by epigallocatechin gallate involves its binding to Fas

被引:114
作者
Hayakawa, S
Saeki, K
Sazuka, M
Suzuki, Y
Shoji, Y
Ohta, T
Kaji, K
Yuo, A
Isemura, M [1 ]
机构
[1] Univ Shizuoka, Sch Food & Nutr Sci, Yada, Shizuoka 4228526, Japan
[2] Univ Shizuoka, Grad Sch Food & Environm Sci, Yada, Shizuoka 4228526, Japan
[3] Int Med Ctr Japan, Inst Res, Dept Hematol, Shinjuku Ku, Tokyo 1628655, Japan
关键词
apoptosis; epigallocatechin gallate (EGCG); Fas; U937; tumor; caspase; 8;
D O I
10.1006/bbrc.2001.5293
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Epigallocatechin gallate (EGCG) is known to induce apoptosis in various types of tumor cells, but the precise mechanism by which EGCG induces apoptosis remains to be elucidated. The Fas-Fas ligand system is one of the major pathways operating in the apoptotic cascade. The aim of this study was to examine the possibility that EGCG-binding to Fas triggers the Fas-mediated apoptosis. The EGCG treatment of human monocytic leukemia U937 cells resulted in elevation of caspase 8 activity and fragmentation of caspase 8. The DNA ladder formation caused by the EGCG treatment was inhibited by the caspase 8 inhibitor. These findings suggested the involvement of the Fas-mediated cascade in the EGCG-induced apoptosis in U937 cells. Affinity chromatography revealed the binding between EGCG and Fas. Thus, the results suggest that EGCG-binding to Fas, presumably on the cell surface, triggers the Fas-mediated apoptosis in U937 cells. (C) 2001 Academic Press.
引用
收藏
页码:1102 / 1106
页数:5
相关论文
共 27 条
  • [1] Cell cycle dysregulation by green tea polyphenol epigallocatechin-3-gallate
    Ahmad, N
    Cheng, PY
    Mukhtar, H
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 275 (02) : 328 - 334
  • [2] Green tea polyphenol epigallocatechin-3-gallate differentially modulates nuclear factor κB in cancer cells versus normal cells
    Ahmad, N
    Gupta, S
    Mukhtar, H
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2000, 376 (02) : 338 - 346
  • [3] Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells
    Ahmad, N
    Feyes, DK
    Nieminen, AL
    Agarwal, R
    Mukhtar, H
    [J]. JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1997, 89 (24): : 1881 - 1886
  • [4] Green tea epigallocatechin gallate shows a pronounced growth inhibitory effect on cancerous cells but not on their normal counterparts
    Chen, ZP
    Schell, JB
    Ho, CT
    Chen, KY
    [J]. CANCER LETTERS, 1998, 129 (02) : 173 - 179
  • [5] Cancer inhibition by green tea
    Fujiki, H
    Suganuma, M
    Okabe, S
    Sueoka, N
    Komori, A
    Sueoka, E
    Kozu, T
    Tada, Y
    Suga, K
    Imai, K
    Nakachi, K
    [J]. MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1998, 402 (1-2) : 307 - 310
  • [6] Inhibition of human caspases by peptide-based and macromolecular inhibitors
    Garcia-Calvo, M
    Peterson, EP
    Leiting, B
    Ruel, R
    Nicholson, DW
    Thornberry, NA
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (49) : 32608 - 32613
  • [7] Apoptosis-inducing activity of high molecular weight fractions of tea extracts
    Hayakawa, S
    Kimura, T
    Saeki, K
    Koyama, Y
    Aoyagi, Y
    Noro, T
    Nakamura, Y
    Isemura, M
    [J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2001, 65 (02) : 459 - 462
  • [8] Hibasami H, 1996, ANTICANCER RES, V16, P1943
  • [9] Sealing effects of (-)-epigallocatechin gallate on protein kinase C and protein phosphatase 2A
    Kitano, K
    Nam, KY
    Kimura, S
    Fujiki, H
    Imanishi, Y
    [J]. BIOPHYSICAL CHEMISTRY, 1997, 65 (2-3) : 157 - 164
  • [10] Antimutagenic and anticarcinogenic activity of tea polyphenols
    Kuroda, Y
    Hara, Y
    [J]. MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 1999, 436 (01) : 69 - 97