Regulation and cloning of microbial chitinase genes

被引:87
作者
Felse, PA [1 ]
Panda, T [1 ]
机构
[1] Indian Inst Technol, Biotechnol Res Ctr, Madras 600036, Tamil Nadu, India
关键词
D O I
10.1007/s002530051374
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A range of chitinase genes from microorganisms have been cloned and the potential uses of these genetically manipulated organisms are being investigated by various researchers. Fungi and yeast are better producers of chitinase than bacteria. Since fungi grow at a slower rate, there have been efforts to clone the fungal chitinase genes into fast-growing bacteria. This review gives a brief survey of recent progress in the regulation and cloning of microbial chitinase genes. Emphasis is placed on the post-translational modification and localization of the recombinant protein in the host. Various amino acid domains are present in this protein. The mode of catalytic activity of the recombinant protein in comparison to the wild-type protein is discussed in the available literature. The different mechanisms involved in the regulation of chitinase genes from various microorganisms is discussed by the researchers. The scope of future research and conclusions yet to be obtained in this particular area are also outlined in this review.
引用
收藏
页码:141 / 151
页数:11
相关论文
共 60 条
[1]  
ANJANIKUMARI J, 1992, ENZYME MICROB TECHNO, V14, P241
[2]  
BINDER A, 1978, BIOTECHNOL BIOENG, V19, P1187
[3]  
BLAAK H, 1995, EUR J BIOCHEM, V229, P132, DOI 10.1111/j.1432-1033.1995.tb20447.x
[4]   CLONING AND EXPRESSION OF A CHITINASE GENE FROM THE HYPERPARASITIC FUNGUS APHANOCLADIUM-ALBUM [J].
BLAISEAU, PL ;
KUNZ, C ;
GRISON, R ;
BERTHEAU, Y ;
BRYGOO, Y .
CURRENT GENETICS, 1992, 21 (01) :61-66
[5]  
BURBERG MB, 1994, APPL MICROBIOL BIOT, V42, P108
[6]   BIOCONVERSION OF SHELLFISH CHITIN WASTES - PROCESS CONCEPTION AND SELECTION OF MICROORGANISMS [J].
CARROAD, PA ;
TOM, RA .
JOURNAL OF FOOD SCIENCE, 1978, 43 (04) :1158-1161
[7]   CHARACTERIZATION OF ECH-42, A TRICHODERMA-HARZIANUM ENDOCHITINASE GENE EXPRESSED DURING MYCOPARASITISM [J].
CARSOLIO, C ;
GUTIERREZ, A ;
JIMENEZ, B ;
VANMONTAGU, M ;
HERRERAESTRELLA, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :10903-10907
[8]  
CHANPEN W, 1997, GENE, V179, P119
[9]   CLONING AND EXPRESSION OF A CHITINASE GENE FROM AEROMONAS-HYDROPHILA IN ESCHERICHIA-COLI [J].
CHEN, JP ;
NAGAYAMA, F ;
CHANG, MC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (08) :2426-2428
[10]   Expression and characterization of the recombinant gene encoding chitinase from Aeromonas caviae [J].
Lin, CS ;
Chen, HC ;
Lin, FP .
ENZYME AND MICROBIAL TECHNOLOGY, 1997, 21 (07) :472-478