Rapid and reproducible radiosynthesis of [18F] FHBG

被引：38

作者：

Ponde, DE ^{[1
]}

Dence, CS ^{[1
]}

Schuster, DP ^{[1
]}

Welch, MJ ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Edward Mallinckrodt Inst Radiol, Div Radiol Sci, St Louis, MO 63110 USA

来源：

NUCLEAR MEDICINE AND BIOLOGY | 2004年 / 31卷 / 01期

关键词：

F-18] FHBG; PET; thymidine kinase; gene therapy; solid phase purification; Oasis (R) HLB; microwave;

D O I：

10.1016/S0969-8051(03)00096-9

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

9-(4-[F-18] Fluoro-3-hydroxymethylbutyl) guanine ([F-18] FHBG), an imaging agent for gene therapy using PET, was prepared in a one-pot, two-step synthesis. Microwave (MW) mediated nucleophilic fluorination of N-2, monomethoxytrityl-9-[4-(tosyl)-3-monomethoxytrityl-methylbutyl] guanine using no-carrier-added [F-18] fluoride, followed by deprotection with hydrochloric acid and HPLC purification, gave [F-18] FHBG. The radiochemical yield (decay corrected) was 12 +/- 5% (n = 35), the synthesis time was 55-60 min, and the radiochemical purity was >99%. The compound was used for lung imaging and was injected into Sprague-Dawley rats previously infected with the herpes simplex virus type 1 thymidine kinase (HSV1-tk) reporter gene. MicroPET imaging showed accumulation confined to the lungs. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：133 / 138

页数：6

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