Inactivation and potential repair of Cryptosporidium parvum following low- and medium-pressure ultraviolet irradiation

被引：61

作者：

Zimmer, JL

Slawson, RM ^{[1
]}

Huck, PM

机构：

[1] Univ Waterloo, Dept Civil Engn, NSERC Chair Water Treatment, Waterloo, ON N2L 3G1, Canada

[2] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada

来源：

WATER RESEARCH | 2003年 / 37卷 / 14期

基金：

加拿大自然科学与工程研究理事会;

关键词：

UV radiation; Cryptosporidium parvum; DNA repair; HCT-8 cell culture; low-pressure UV lamps; medium-pressure UV lamps;

D O I：

10.1016/S0043-1354(03)00238-0

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study investigated the level of inactivation and the potential for Cryptosporidium parvum to repair following low doses (1 and 3 mJ/cm(2)) of ultraviolet (UV) irradiation from both low- and medium-pressure UV lamps. Cryptosporidium parvum oocysts suspended in phosphate buffered saline were exposed to UV using a bench-scale collimated beam apparatus. Oocyst suspensions were incubated at 5degreesC or 25degreesC under light and dark conditions up to 120 h (5 days) following exposure to UV irradiation, to examine photoreactivation and dark repair potential, respectively. Cryptosporidium parvum infectivity was determined throughout the incubation period using an HCT-8 cell culture and an antibody staining procedure for detection. No detectable evidence of repair was observed after incubation under light or dark conditions following either LP or MP UV lamp irradiation. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：3517 / 3523

页数：7

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