A virus-encoded inhibitor that blocks RNA interference in mammalian cells

被引:136
作者
Sullivan, CS
Ganem, D
机构
[1] Univ Calif San Francisco, Howard Hughes Med Inst, Dept Microbiol & Immunol, GW Hooper Res Fdn,Med Ctr, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Howard Hughes Med Inst, Dept Med, GW Hooper Res Fdn,Med Ctr, San Francisco, CA 94143 USA
关键词
D O I
10.1128/JVI.79.12.7371-7379.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Nodamura virus (NoV) is a small RNA virus that is infectious for insect and mammalian hosts. We have developed a highly sensitive assay of RNA interference (RNAi) in mammalian cells that shows that the NoV B2 protein functions as an inhibitor of RNAi triggered by either short hairpin RNAs or small interfering RNAs. In the cell, NoV B2 binds to pre-Dicer substrate RNA and RNA-induced silencing complex (RISC)-processed RNAs and inhibits the Dicer cleavage reaction and, potentially, one or more post-Dicer activities. In vitro, NoV B2 inhibits Dicer-mediated RNA cleavage in the absence of any other host factors and specifically binds double-stranded RNAs corresponding in structure to Dicer substrates and products. Its abilities to bind to Dicer precursor and post-Dicer RISC-processed RNAs suggest a mechanism of inhibition that is unique among known viral inhibitors of RNAi.
引用
收藏
页码:7371 / 7379
页数:9
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