Effects of 7 hemoglobin variants on the measurement of glycohemoglobin by 14 analytical methods

被引:31
作者
Lee, Seung-Tae
Weykamp, Cas W.
Lee, Yong-Wha
Kim, Jong-Won
Ki, Chang-Seok
机构
[1] Sungkyunkwan Univ, Sch Med, Dept Lab Med & Genet, Samsung Med Ctr, Seoul 135710, South Korea
[2] Queen Beatrix Hosp, Dept Clin Chem, Winterswijk, Netherlands
[3] Soonchunhyang Univ, Bucheon Hosp, Dept Lab Med & Genet, Gyeonggi Do, South Korea
[4] Soonchunhyang Univ, Coll Med, Gyeonggi Do, South Korea
关键词
D O I
10.1373/clinchem.2007.093963
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: Hemoglobin variants (Hb(VAR)) are not uncommon in the Korean population, with Hb G-Coushatta and Hb Queens being the 2 most common Hb(VAR). Hb G-Coushatta is also the most common Hb(VAR) in Chinese people from the Silk Road region, as well as in some North American Indian tribes. However, data are scarce on the effect of these Hb(VAR) on the different methods used for analyzing HbA(1c). Methods: Specimens from 24 individuals with 7 Hb(VAR) (Hb G-Coushatta, Hb Queens, Hb G-Hsi-Tsou, Hb Ube-4, Hb G-Waimanalo, Hb Inglewood, and Hb Bologna-St.Orsola) were collected and tested using the International Federation of Clinical Chemistry primary reference method as well as 14 routine HbA(1c) assay methods. Results: Hb G-Coushatta showed a clinically significant effect on the measured HbA(1c), particularly when analysis was performed with ion-exchange HPLC methods with short elution times. This interference could be resolved by measuring the HbA(1c) using other methods such as HPLC with a long elution time, immunoassay, boronate affinity chromatography, and enzymatic assay. Hb Queens showed a clinically significant difference, defined as a >10% deviation from regression lines, in results from the 2 HPLC methods but not in the other methods. The remaining 5 rare Hb(VAR) showed different HbA(1c) results in the different assays. Conclusion: Hb G-Coushatta, Hb Queens, and other rare Hb(VAR) can interfere with glycohemoglobin assays, including ion-exchange HPLC methods with short elution times, but the interference can be resolved using other unaffected methods. It is important to identify these Hb(VAR) through a careful inspection of the chromatograms and apply other noninterfering methods for accurate measurements of the HbA(1c). (C) 2007 American Association for Clinical Chemistry.
引用
收藏
页码:2202 / 2205
页数:4
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