DLA-DRB1 and DLA-DQB1 histocompatibility typing by PCR-SSCP and sequencing

被引：68

作者：

Wagner, JL

Works, JD

Storb, R

机构：

[1] Fred Hutchinson Canc Res Ctr, Program Transplantat Biol, Div Clin Res, Seattle, WA 98109 USA

[2] Univ Washington, Sch Med, Dept Med, Seattle, WA USA

来源：

TISSUE ANTIGENS | 1998年 / 52卷 / 04期

关键词：

DLA-DQB1; DLA-DRB1; histocompatibility typing; PCR-SSCP;

D O I：

10.1111/j.1399-0039.1998.tb03063.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The dog has been an important model for solid organ and hematopoietic stem cell transplantation for over 30 years. Fundamental to the continuing usage of the model is the development of molecular-based histocompatibility typing of donors and recipients. Previous histocompatibility typing methods used in the dog have not been precise enough to identify dog leukocyte antigen (DLA)-matched unrelated dogs. This study was undertaken to begin the process of identifying DLA-matched unrelated dogs. In this study polymerase chain reaction-single-stranded conformational polymorphism is used to separate alleles thereby allowing sequenced-based typing of the two most polymorphic class II genes described to date in the dog - DLA-DRB1 and DLA-DQB1.

引用

页码：397 / 401

页数：5

共 21 条

[1] COMPLETE SUBTYPING OF THE HLA-A LOCUS BY SEQUENCE-SPECIFIC AMPLIFICATION FOLLOWED BY DIRECT SEQUENCING OR SINGLE-STRAND CONFORMATION POLYMORPHISM ANALYSIS [J].