Age-related decline of perforin expression in human cytotoxic T lymphocytes and natural killer cells

被引:101
作者
Rukavina, D
Laskarin, G
Rubesa, G
Strbo, N
Bedenicki, I
Manestar, D
Glavas, M
Christmas, SE
Podack, ER
机构
[1] Univ Rijeka, Fac Med, Dept Physiol & Immunol, HR-51000 Rijeka, Croatia
[2] Univ Rijeka, Clin Hosp Ctr, Dept Otorhinolaryngol, HR-51000 Rijeka, Croatia
[3] Clin Hosp Ctr Pula, Pula, Croatia
[4] Univ Liverpool, Dept Immunol, Liverpool L69 3BX, Merseyside, England
[5] Univ Miami, Dept Microbiol & Immunol, Miami, FL 33152 USA
关键词
D O I
10.1182/blood.V92.7.2410.2410_2410_2420
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In this study a flow cytometric technique for detecting cytoplasmic perforin (P) has been used to quantify age-related changes in perforin expression in human peripheral blood lymphocytes (PBL). Proportions of P+ lymphocytes increased after birth, but declined rapidly after the age of 70 years. This was true for both T cells and CD16(+) and CD56(+) natural killer (NK) cells. Children showed in addition to high levels of perforin positive CD8(+) cells a much higher proportion of CD4(+)P(+) cells than the other age groups. In elderly individuals there was also a highly significant reduction in mean levels of perforin per cell as compared with all other groups (P<.05 to .001). Adult women had consistently higher mean levels of perforin per cell than adult men for all P+ cell phenotypes. Functional tests clearly showed the deficiency in early spontaneous cytotoxic potential of PBL from elderly persons due to relative P deficiency, which can be corrected by stimulation of cytolytic cells with target cells and interleukin-2 (IL-2). The deficiency in cytolytic activity on the contact with target cells may have implications for antiviral and antitumor immunity in elderly persons. (C) 1998 by The American Society of Hematology.
引用
收藏
页码:2410 / 2420
页数:11
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