Protein affinity map of chemical space

被引：22

作者：

Kauvar, LM ^{[1
]}

Villar, HO ^{[1
]}

Sportsman, JR ^{[1
]}

Higgins, DL ^{[1
]}

Schmidt, DE ^{[1
]}

机构：

[1] Terrapin Technol Inc, S San Francisco, CA 94080 USA

来源：

JOURNAL OF CHROMATOGRAPHY B | 1998年 / 715卷 / 01期

关键词：

affinity fingerprinting; chemical space; proteins;

D O I：

10.1016/S0378-4347(98)00045-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Affinity fingerprinting is a quantitative method for mapping chemical space based on binding preferences of compounds for a reference panel of proteins. An effective reference panel of <20 proteins can be empirically selected which shows differential interaction with nearly all compounds. By using this map to iteratively sample the chemical space, identification of active ligands from a library of 30 000 candidate compounds has been accomplished for a wide spectrum of specific protein targets. In each case, <200 compounds were directly assayed against the target. Further, analysis of the fingerprint database suggests a strategy for effective selection of affinity chromatography ligands and scaffolds for combinatorial chemistry. With such a system, the large numbers of potential therapeutic targets emerging from genome research can be categorized according to ligand binding properties, complementing sequence based classification. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：93 / 102

页数：10

共 29 条

[1] A COMPARATIVE TEST OF 15 COMPOUNDS AGAINST ALL KNOWN HUMAN RHINOVIRUS SEROTYPES AS A BASIS FOR A MORE RATIONAL SCREENING-PROGRAM [J].