Immobilized culture of nonadherent cells on an oleyl poly(ethylene glycol) ether-modified surface

被引：72

作者：

Kato, K ^{[1
]}

Umezawa, K ^{[1
]}

Funeriu, DP ^{[1
]}

Miyake, M ^{[1
]}

Miyake, J ^{[1
]}

Nagamune, T ^{[1
]}

机构：

[1] Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan

来源：

BIOTECHNIQUES | 2003年 / 35卷 / 05期

关键词：

D O I：

10.2144/03355rr01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microarrays of living cells are an emerging toot in systems such as reverse transfection. These studies are limited to adherent cells partly because of the difficulty of cell immobilization. Using a newly developed reagent, the biocompatible anchor for membrane (BAM), we show herein the rapid and strong attachment of living nonadherent cells and adherent cells on BAM-modified surfaces. Normal cellular growth was observed for over 7 days on BAM-modified surfaces. We expect this methodology to greatly expand the scope of current cell microarray technology.

引用

页码：1014 / +

页数：6

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