The detection of Salmonella serovars from animal feed and raw chicken using a combined immunomagnetic separation and ELISA method

被引：36

作者：

Mansfield, LP ^{[1
]}

Forsythe, SJ ^{[1
]}

机构：

[1] Nottingham Trent Univ, Dept Life Sci, Nottingham NG11 8NS, England

来源：

FOOD MICROBIOLOGY | 2001年 / 18卷 / 04期

关键词：

D O I：

10.1006/fmic.2001.0416

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The immunomagnetic separation technique was used in conjunction with an ELISA-based detection system to recover Salmonella enteritidis, Salmonella typhimurium and Salmonella virchow (2-2 x 10(3) cfu 25 g(-1)) from raw chicken and animal feed. The detection time was less than 27 h and included the conventional pre-enrichment (18 h) step in buffered peptone water and an enrichment step (6 h) in glucose nutrient broth. In contrast, the conventional method using Rappaport-Vassiliades enrichment broth and selective plating on XLD agar required 72-96 h. (C) 2001 Academic Press.

引用

页码：361 / 366

页数：6

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