Non-live probiotic bacteria and their fermentation products can be used in milk-based formula intended for healthy infants. The effects of a milk formula fermented with Bifidobacterium breve and Streptococcus thermophilus and heated/dehydrated to inactivate the micro-organisms have been reported over the last few years to decrease the intestinal permeability to macromolecules in experimental animals in vivo and more recently to down-regulate inflammatory condition in vitro. Feeding guinea-pigs with such dehydrated fermented milk reinforced the intestinal barrier resistance to food proteins (HRP, beta-lactoglobulin). In addition, the products secreted by bacteria were capable of inhibiting the lipopolysaccharide (LPS)-induced TNF-alpha secretion by human peripheral mononuclear blood cells. The active secretion products were resistant to digestive enzymes and their anti-inflammatory properties were preserved after transepithelial transport across the filter-grown intestinal epithelial cell line, especially in inflammatory conditions. The binding of LPS to monocytes as well as NF kappa B nuclear translocation leading to pro-inflammatory cytokine transcription were inhibited by bacteria-culture supernatants. Conclusion: B. breve and S. thermophilus used as non-live micro-organisms in fermented infant formula seem to induce a reduction in macromolecular absorption and release metabolites exerting an anti-TNF-alpha effect, which persists after intestinal transport. Thus, specific lactic acid bacteria and their metabolites seem to affect positively the intestinal function.
机构:
Univ Calif San Diego, Dept Med, Sch Med, Med Ctr 8414, San Diego, CA 92103 USAUniv Calif San Diego, Dept Med, Sch Med, Med Ctr 8414, San Diego, CA 92103 USA
Resta-Lenert, S
;
Barrett, KE
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机构:
Univ Calif San Diego, Dept Med, Sch Med, Med Ctr 8414, San Diego, CA 92103 USAUniv Calif San Diego, Dept Med, Sch Med, Med Ctr 8414, San Diego, CA 92103 USA
机构:
Univ Calif San Diego, Dept Med, Sch Med, Med Ctr 8414, San Diego, CA 92103 USAUniv Calif San Diego, Dept Med, Sch Med, Med Ctr 8414, San Diego, CA 92103 USA
Resta-Lenert, S
;
Barrett, KE
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Diego, Dept Med, Sch Med, Med Ctr 8414, San Diego, CA 92103 USAUniv Calif San Diego, Dept Med, Sch Med, Med Ctr 8414, San Diego, CA 92103 USA