Proteomic analysis of outer membrane vesicles derived from Pseudomonas aeruginosa

被引：192

作者：

Choi, Dong-Sic ^{[1
,2
]}

Kim, Dae-Kyum ^{[1
,2
]}

Choi, Seng Jin ^{[1
,2
]}

Lee, Jaewook ^{[1
,2
]}

Choi, Jun-Pyo ^{[1
,2
]}

Rho, Sangchul ^{[3
]}

Park, Sang-Hyun ^{[4
]}

Kim, Yoon-Keun ^{[1
,2
]}

Hwang, Daehee ^{[5
]}

Gho, Yong Song ^{[1
,2
]}

机构：

[1] Pohang Univ Sci & Technol, Dept Life Sci, Pohang, South Korea

[2] Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang, South Korea

[3] Pohang Univ Sci & Technol, Sch Interdisciplinary Biosci & Bioengn, Pohang, South Korea

[4] Seoul Natl Univ, Dept Biol Sci, Seoul, South Korea

[5] Pohang Univ Sci & Technol, Dept Chem Engn, Pohang, South Korea

来源：

PROTEOMICS | 2011年 / 11卷 / 16期

关键词：

Microbiology; Outer membrane vesicles; Pseudomonas aeruginosa; Vaccine; GRAM-NEGATIVE BACTERIA; INNATE IMMUNE-RESPONSE; CYSTIC-FIBROSIS; SAMPLE PREPARATION; MASS-SPECTROMETRY; BETA-LACTAMASE; PROTEINS; IDENTIFICATIONS; PURIFICATION; INFECTIONS;

D O I：

10.1002/pmic.201000212

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Pseudomonas aeruginosa, an opportunistic human bacterial pathogen, constitutively secretes outer membrane vesicles (OMVs) into the extracellular milieu. Although recent progress has revealed that OMVs are essential for pathogenesis of P. aeruginosa, their proteins have not been comprehensively analyzed so far. In this study, we identified 338 vesicular proteins with high confidence by five separate LC-MS/MS analyses. This global proteome profile provides a basis for future studies to elucidate the pathological functions of OMVs from P. aeruginosa.

引用

页码：3424 / 3429

页数：6

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