A TEMPO-conjugated fluorescent probe for monitoring mitochondrial redox reactions

被引：57

作者：

Hirosawa, Shota ^{[1
]}

Arai, Satoshi ^{[2
]}

Takeoka, Shinji ^{[1
]}

机构：

[1] Waseda Univ TWIns, Grad Sch Adv Sci & Engn, Dept Life Sci & Med Biosci, Tokyo 1628480, Japan

[2] Waseda Univ, Consolidated Res Inst Adv Sci & Med Care, Tokyo 1620041, Japan

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 40期

关键词：

HYDROGEN-PEROXIDE; LIVING CELLS; APOPTOSIS; DYSFUNCTION; NITROXIDE; UBIQUINOL; RADICALS; DISEASES;

D O I：

10.1039/c2cc30603d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a mitochondrial targeted redox probe (MitoRP) that comprises a nitroxide radical (TEMPO) moiety and coumarin 343. Using isolated mitochondria in the presence/absence of substrates and inhibitors of oxidative phosphorylation, we demonstrated that MitoRP is a useful probe to monitor the electron flow associated with complex I.

引用

页码：4845 / 4847

页数：3

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