QTL mapping of volatile compounds in ripe apples detected by proton transfer reaction-mass spectrometry

被引:59
作者
Zini, E
Biasioli, F
Gasperi, F
Mott, D
Aprea, E
Märk, TD
Patocchi, A
Gessler, C
Komjanc, M
机构
[1] Ist Agr S Michele allAdige, SafeCrop Ctr, I-38010 San Michele All Adige, Italy
[2] Univ Innsbruck, Inst Ionenphys, A-6020 Innsbruck, Austria
[3] Univ Bratislava, Dept Plasmaphys, SK-84248 Bratislava, Slovakia
[4] Swiss Fed Inst Technol, Inst Plant Sci, CH-8092 Zurich, Switzerland
基金
奥地利科学基金会;
关键词
linkage map; Malus; on-line headspace analysis; PTR-MS; QTLs; Volatile Organic Compounds;
D O I
10.1007/s10681-005-1645-9
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The availability of genetic linkage maps enables the detection and analysis of QTLs contributing to quality traits of the genotype. Proton Transfer Reaction Mass Spectrometry (PTR-MS), a relatively novel spectrometric technique, has been applied to measure the headspace composition of the Volatile Organic Compounds (VOCs) emitted by apple fruit genotypes of the progeny 'Fiesta' x 'Discovery'. Fruit samples were characterised by their PTR-MS spectra normalised to total area. QTL analysis for all PTR-MS peaks was carried out and 10 genomic regions associated with the peaks at m/z = 28, 43, 57, 61, 103, 115 and 145 were identified (LOD > 2.5). We show that it is possible to find quantitative trait loci (QTLs) related to PTR-MS characterisation of the headspace composition of single whole apple fruits indicating the presence of a link between molecular characterisation and PTR-MS data. We provide tentative information on the metabolites related to the detected QTLs based on available chemical information. A relation between apple skin colour and peaks related to carbonyl compounds was established.
引用
收藏
页码:269 / 279
页数:11
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