Metagenomic gene discovery: past, present and future

被引：168

作者：

Cowan, D ^{[1
]}

Meyer, Q ^{[1
]}

Stafford, W ^{[1
]}

Muyanga, S ^{[1
]}

Cameron, R ^{[1
]}

Wittwer, P ^{[1
]}

机构：

[1] Univ Western Cape, Dept Biotechnol, Adv Res Ctr Appl Microbiol, ZA-7535 Bellville, South Africa

来源：

TRENDS IN BIOTECHNOLOGY | 2005年 / 23卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1016/j.tibtech.2005.04.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

It is now widely accepted that the application of standard microbiological methods for the recovery of microorganisms from the environment has had limited success in providing access to the true extent of microbial biodiversity. It follows that much of the extant microbial genetic diversity (collectively termed the metagenome) remains unexploited, an issue of considerable relevance to a wider understanding of microbial communities and of considerable importance to the biotechnology industry. The recent development of technologies designed to access this wealth of genetic information through environmental nucleic acid extraction has provided a means of avoiding the limitations of culture-dependent genetic exploitation.

引用

页码：321 / 329

页数：9

共 93 条

[1] Ab initio gene identification:: prokaryote genome annotation with GeneScan and GLIMMER [J].