Human WIPI-1 puncta-formation: A novel assay to assess mammalian autophagy

被引：129

作者：

Proikas-Cezanne, Tassula

Ruckerbauer, Sabine

Stierhof, York-Dieter

Berg, Carolin

Nordheim, Alfred

机构：

[1] Univ Tubingen, Dept Mol Biol, Autophagy Lab, D-72076 Tubingen, Germany

[2] Univ Tubingen, Microscopy Unit, ZMBP, Tubingen, Germany

[3] Univ Tubingen, Dept Mol Biol, Tubingen, Germany

来源：

FEBS LETTERS | 2007年 / 581卷 / 18期

关键词：

WIPI-1; Atg18; LC3; PI(3)P; autophagy;

D O I：

10.1016/j.febslet.2007.06.040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Autophagy depends on the activity of phosphoinositide-3 kinase class III to generate PI(3)P. We identified the human WIPI protein family of PI(3)P-binding factors and showed that WIPI-1 (Atg18) is linked to autophagy in human cells. Induction of autophagy by rapamycin, gleevec, thapsigargin and amino acid deprivation led to an accumulation of WIPI-1 at LC3-positive membrane structures (WIPI-1 puncta-formation), suggested to represent autophagosomal isolation membranes. WIPI-1 puncta-formation is inhibited by wortmannin and LY294002, and PI(3)P-binding-deficient WIPI-1 is puncta-formation-incompetent. Quantification of WIPI-1 puncta should be suitable to assay mammalian autophagy. (c) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

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收藏

页码：3396 / 3404

页数：9

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