Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins

被引：74

作者：

Kurtzman, AL ^{[1
]}

Govindarajan, S ^{[1
]}

Vahle, K ^{[1
]}

Jones, JT ^{[1
]}

Heinrichs, V ^{[1
]}

Patten, PA ^{[1
]}

机构：

[1] Maxygen Inc, Redwood City, CA 94063 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(00)00228-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent developments in directed evolution technologies combined with innovations in robotics and screening methods have revolutionized protein engineering. These methods are being applied broadly to many fields of biotechnology, including chemical engineering, agriculture and human therapeutics. More specifically, DNA shuffling and other methods of genetic recombination and mutation have resulted in the improvement of proteins of therapeutic interest. Optimizing genetic diversity and fitness through iterative directed evolution will accelerate improvements in engineered protein therapeutics.

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收藏

页码：361 / 370

页数：10

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