Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins

被引：74

作者：

Kurtzman, AL ^{[1
]}

Govindarajan, S ^{[1
]}

Vahle, K ^{[1
]}

Jones, JT ^{[1
]}

Heinrichs, V ^{[1
]}

Patten, PA ^{[1
]}

机构：

[1] Maxygen Inc, Redwood City, CA 94063 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 04期

关键词：

D O I：

10.1016/S0958-1669(00)00228-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent developments in directed evolution technologies combined with innovations in robotics and screening methods have revolutionized protein engineering. These methods are being applied broadly to many fields of biotechnology, including chemical engineering, agriculture and human therapeutics. More specifically, DNA shuffling and other methods of genetic recombination and mutation have resulted in the improvement of proteins of therapeutic interest. Optimizing genetic diversity and fitness through iterative directed evolution will accelerate improvements in engineered protein therapeutics.

引用

收藏

页码：361 / 370

页数：10

相关论文

共 67 条

[51] Basal insulin glargine (HOE 901) versus NPH insulin in patients with type 1 diabetes on multiple daily insulin regimens [J].

Rosenstock, J ;

Park, G ;

Zimmerman, J .

DIABETES CARE, 2000, 23 (08) :1137-1142

[52]

Sandberg H, 2001, THROMB HAEMOSTASIS, V85, P93

[53] Molecular breeding of carotenoid biosynthetic pathways [J].

Schmidt-Dannert, C ;

Umeno, D ;

Arnold, FH .

NATURE BIOTECHNOLOGY, 2000, 18 (07) :750-753

[54] Libraries of hybrid proteins from distantly related sequences [J].

Sieber, V ;

Martinez, CA ;

Arnold, FH .

NATURE BIOTECHNOLOGY, 2001, 19 (05) :456-460

[55] Molecular breeding of viruses [J].

Soong, NW ;

Nomura, L ;

Pekrun, K ;

Reed, M ;

Sheppard, L ;

Dawes, G ;

Stemmer, WPC .

NATURE GENETICS, 2000, 25 (04) :436-439

[56] MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF HUMAN GLUCOCEREBROSIDASE CDNA [J].

SORGE, J ;

WEST, C ;

WESTWOOD, B ;

BEUTLER, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (21) :7289-7293

[57] RAPID EVOLUTION OF A PROTEIN IN-VITRO BY DNA SHUFFLING [J].

STEMMER, WPC .

NATURE, 1994, 370 (6488) :389-391

[58] DNA SHUFFLING BY RANDOM FRAGMENTATION AND REASSEMBLY - IN-VITRO RECOMBINATION FOR MOLECULAR EVOLUTION [J].

STEMMER, WPC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (22) :10747-10751

[59] Different structural requirements for plasminogen activator inhibitor 1 (PAI-1) during latency transition and proteinase inhibition as evidenced by phage-displayed hypermutated PAI-1 libraries [J].

Stoop, AA ;

Eldering, E ;

Dafforn, TR ;

Read, RJ ;

Pannekoek, H .

JOURNAL OF MOLECULAR BIOLOGY, 2001, 305 (04) :773-783

[60] STRUCTURE OF PLASMINOGEN-ACTIVATOR INHIBITOR-1 (PAI-1) AND ITS FUNCTION IN FIBRINOLYSIS - AN UPDATE [J].

VANMEIJER, M ;

PANNEKOEK, H .

FIBRINOLYSIS, 1995, 9 (05) :263-276

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