Molecular characterization of acute leukemias by use of microarray technology

被引:101
作者
Kohlmann, A
Schoch, C
Schnittger, S
Dugas, M
Hiddemann, W
Kern, W
Haferlach, T
机构
[1] Univ Munich, Dept Internal Med 3, Dept Leukemia Diagnost, D-81377 Munich, Germany
[2] Univ Munich, Dept Med Infomat Biometr & Epidemiol, Munich, Germany
关键词
D O I
10.1002/gcc.10225
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Accurate subclassification of leukemia and the identification of prognostic determinants are essential to guide therapy and to improve patients' outcome. According to present standards, pre-therapeutic assessment depends on a combination of different methods. We aimed to expand the molecular characterization of different acute leukemia subtypes to identify new genome-wide diagnostic markers. Total RNA from 90 adult patients suffering from acute lymphoblastic leukemia (ALL, n = 25) and acute myeloid leukemia (AML, n = 65) was extracted at diagnosis and high density oligonucleotide microarrays were used to analyze the expression profiles of 12,000/22,000 genes in all specimens (Affymetrix U95Av2/U133A). All cases were thoroughly characterized by individual combinations of cytomorphology, cytogenetics, multiparameter immunophenotyping, and molecular genetics. The expression signature of a small set of differentially expressed genes was sufficient to accurately discriminate eight clinically relevant acute leukemia subgroups. Underlying chromosomal aberrations or immunophenotypical characteristics were strictly correlated with a distinct gene expression pattern for AML with t(8;21), t(15;17), t(11q23)/MLL, or inv(16) as well as for precursor B-ALL with t(9;22), t(8;14), or t(11q23)/MLL and precursor T-ALL. These data support a possible future application of microarray technology for classification of the acute leukemias. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:396 / 405
页数:10
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