Differential gene expression in functional classes of interstitial cells of Cajal in murine small intestine

被引:97
作者
Chen, Hui [1 ]
Oerdoeg, Tamas [1 ]
Chen, Junwei [1 ]
Young, David L. [1 ]
Bardsley, Michael R. [1 ]
Redelman, Doug [1 ,2 ]
Ward, Sean M. [1 ]
Sanders, Kenton M. [1 ]
机构
[1] Univ Nevada, Sch Med, Dept Physiol & Cell Biol, Reno, NV 89557 USA
[2] Univ Nevada, Cytometry Ctr, Reno, NV USA
关键词
Affymetrix Mouse Genome 430.2 GeneChips; Bioconductor; enteric nervous system; ion channel; signal transduction;
D O I
10.1152/physiolgenomics.00113.2007
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Interstitial cells of Cajal (ICC) have important functions in regulation of motor activity in the gastrointestinal tract. In murine small intestine, ICC are gathered in the regions of the myenteric plexus (ICC-MY) and the deep muscular plexus (ICC-DMP). These two classes of ICC have different physiological functions. ICC-MY are pacemaker cells and generate the slow-wave electrical rhythmicity of gastrointestinal organs. ICC-DMP form synaptic connections with the varicose nerve terminals of enteric motor neurons and are involved in reception and transduction of motor neurotransmission. Gene expression underlying specific functions of ICC classes is incompletely understood. In the present study, we used recently developed highly selective techniques to isolate the two functional ICC classes from enzymatically dispersed intestinal muscles by fluorescence-activated cell sorting. The transcriptomes of ICC-MY and ICC-DMP were investigated using oligonucleotide microarray analysis. Differential expression of functional groups of genes defined by standard gene ontology terms was also studied. There were substantial numbers of genes expressed more abundantly in ICC than in the tunica muscularis, and we also detected marked phenotypic differences between ICC-MY and ICC-DMP. Notably, genes related to cell junction, process guidance, and vesicle trafficking were upregulated in ICC. Consistent with their specific functions, metabolic and Ca2+ transport genes were relatively upregulated in ICC-MY, whereas genes for signaling proteins involved in transduction of neurotransmitter functions were relatively upregulated in ICC-DMP. Our results may lead to the identification of novel biomarkers for ICC and provide directions for further studies designed to understand ICC function in health and disease.
引用
收藏
页码:492 / 509
页数:18
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