Postmortem changes in the level of brain proteins

被引:126
作者
Fountoulakis, M
Hardmeier, R
Höger, H
Lubec, G
机构
[1] Univ Vienna, Dept Pediat, A-1090 Vienna, Austria
[2] F Hoffmann La Roche & Co Ltd, Pharmaceut Res, Genomics Technol, CH-4002 Basel, Switzerland
关键词
rat brain; postmortem protein changes; two-dimensional electrophoresis; matrix-assisted laser desorption ionization mass spectroscopy;
D O I
10.1006/exnr.2000.7529
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
A systematic study on postmortem changes of brain proteins has not been performed so far and information is limited to basic principles of specific or nonspecific proteolysis or proteolysis of individual proteins. We studied protein level alterations in rat brain of animals kept at 23 degreesC for several postmortem times up to 72 h. Brain tissue protein extracts were analyzed by two-dimensional electrophoresis and the proteins with different levels were identified by matrix-assisted laser desorption ionization mass spectrometry. The changes observed mainly concerned structural proteins and enzymes. The levels of dihydropyrimidinase-related protein-a decreased within 6 h and two new spots were detected representing shorter forms of the protein. Most of the other alterations appeared about 48 h postmortem. The most significant were reduced levels of neurofilament, alpha -internexin, synaptosomal-associated protein 25, glial fibrillary acidic protein, heat shock proteins, and dynamin-1; increased levels of 14-3-3 proteins and spectrin; and generation of shorter forms of certain proteins, such as tubulins, actin, and serum albumin. The results may be useful in neuropathology and brain protein studies. (C) 2001 Academic Press.
引用
收藏
页码:86 / 94
页数:9
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