Novel method using a laser scanning cytometer for detection of Mycobacteria in clinical samples

被引：17

作者：

Pina-Vaz, C

Costa-Oliveira, S

Rodrigues, AG

Salvador, A

机构：

[1] Univ Porto, Fac Med, Dept Microbiol, P-4200 Oporto, Portugal

[2] Univ Porto, IPATIMUP, Inst Pathol & Mol Immunol, P-4200 Oporto, Portugal

[3] Hosp Sao Joao, Dept Microbiol, Oporto, Portugal

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 02期

关键词：

D O I：

10.1128/JCM.42.2.906-908.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In order to evaluate the capacity of laser scanning cytometry (LSC) to detect acid-fast bacilli directly on clinical samples, a comparison between Kinyoun-stained smears analyzed under light microscopy and propidium iodide-auramine-stained smears analyzed by LSC was performed. The results were compared with those for culture on BACTEC MGIT 960. LSC is a new, reliable methodology to detect Mycobacteria.

引用

页码：906 / +

页数：4

共 14 条

[1]

AVAREZBARRIENTO.J, 2000, CLIN MICROBIOL REV, V13, P167

[2]

Bedner E, 1998, CYTOMETRY, V33, P1, DOI 10.1002/(SICI)1097-0320(19980901)33:1<1::AID-CYTO1>3.0.CO

[3]

2-P

[4]

BISHOP P J, 1970, Tubercle, V51, P196