Charge state and adduct reduction in electrospray ionization-mass spectrometry using solvent vapor exposure

被引：59

作者：

Hopper, Jonathan T. S. ^{[1
]}

Sokratous, Kleitos ^{[1
]}

Oldham, Neil J. ^{[1
]}

机构：

[1] Univ Nottingham, Sch Chem, Nottingham NG7 2RD, England

来源：

ANALYTICAL BIOCHEMISTRY | 2012年 / 421卷 / 02期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Protein mass spectrometry; Protein complexes; Noncovalent interactions; PROTEIN-LIGAND COMPLEXES; GAS-PHASE; ATMOSPHERIC-PRESSURE; SPRAY IONIZATION; ION; DISSOCIATION;

D O I：

10.1016/j.ab.2011.10.034

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The benefits of lowering protein ion charge states in electrospray ionization (ESI) have attracted recent interest. We describe a simple approach to decrease protein charge states by exposure of electrospray droplets to neutral solvent vapor such as acetonitrile. The technique allows detection of weak noncovalent complexes, provides preferred charge states for tandem mass spectrometry (MS/MS) dissociation of protein complexes, and has the added benefit of reducing common adducts, such as alkali metals, without the addition of solution additives or the requirement for a secondary spray. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：788 / 790

页数：3

共 33 条

[1] Gas Phase Stabilization of Noncovalent Protein Complexes Formed by Electrospray Ionization [J].

Bagal, Dhanashri ;

Kitova, Elena N. ;

Liu, Lan ;

El-Hawiet, Amr ;

Schnier, Paul D. ;

Klassen, John S. .

ANALYTICAL CHEMISTRY, 2009, 81 (18) :7801-7806

[2] Mass spectrometry of macromolecular assemblies: preservation and dissociation [J].

Benesch, JL ;

Robinson, CV .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2006, 16 (02) :245-251

[3] Decharging of globular proteins and protein complexes in electrospray [J].

Catalina, MI ;

van den Heuvel, RHH ;

van Duijn, E ;

Heck, AJR .

CHEMISTRY-A EUROPEAN JOURNAL, 2005, 11 (03) :960-968

[4] Determination of ligand-protein dissociation constants by electrospray mass spectrometry-based diffusion measurements [J].

Clark, SM ;

Konermann, L .

ANALYTICAL CHEMISTRY, 2004, 76 (23) :7077-7083

[5] Characterization of intrinsically disordered proteins with electrospray ionization mass spectrometry: Conformational heterogeneity of α-synuclein [J].

Frimpong, Agya K. ;

Abzatimov, Rinat R. ;

Uversky, Vladimir N. ;

Kaltashov, Igor A. .

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 2010, 78 (03) :714-722

[6] Mass Spectrometry Defines the Stoichiometry of Ribosomal Stalk Complexes across the Phylogenetic Tree [J].

Gordiyenko, Yuliya ;

Videler, Hortense ;

Zhou, Min ;

Mckay, Adam R. ;

Fucini, Paola ;

Biegel, Eva ;

Mueller, Volker ;

Robinson, Carol V. .

MOLECULAR & CELLULAR PROTEOMICS, 2010, 9 (08) :1774-1783

[7] Mean charge state and charge state distribution of proteins as structural probes.: An electrospray ionisation mass spectrometry study of lysozyme and ribonuclease A [J].

Halgand, F ;

Laprévote, O .

EUROPEAN JOURNAL OF MASS SPECTROMETRY, 2001, 7 (06) :433-439

[8] Alkali Metal Cation-Induced Destabilization of Gas-Phase Protein-Ligand Complexes: Consequences and Prevention [J].

Hopper, Jonathan T. S. ;

Oldham, Neil J. .

ANALYTICAL CHEMISTRY, 2011, 83 (19) :7472-7479

[9] Mechanism of charging and supercharging molecules in electrospray ionization [J].

Iavarone, AT ;

Williams, ER .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (08) :2319-2327

[10] Supercharged protein and peptide lone formed by electrospray ionization [J].

Iavarone, AT ;

Jurchen, JC ;

Williams, ER .

ANALYTICAL CHEMISTRY, 2001, 73 (07) :1455-1460

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