Gas Phase Stabilization of Noncovalent Protein Complexes Formed by Electrospray Ionization

被引：60

作者：

Bagal, Dhanashri ^{[1
]}

Kitova, Elena N. ^{[2
,3
]}

Liu, Lan ^{[2
,3
]}

El-Hawiet, Amr ^{[2
,3
]}

Schnier, Paul D. ^{[1
]}

Klassen, John S. ^{[2
,3
]}

机构：

[1] Amgen Inc, Mol Struct, Thousand Oaks, CA 91320 USA

[2] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

[3] Univ Alberta, Alberta Ingenu Ctr Carbohydrate Sci, Edmonton, AB T6G 2G2, Canada

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 18期

基金：

加拿大自然科学与工程研究理事会;

关键词：

MASS-SPECTROMETRY; LIGAND-BINDING; DISCHARGE;

D O I：

10.1021/ac900611a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The use of gas phase additives to stabilize noncovalent protein complexes in electrospray ionization mass spectrometry (ES-MS) is demonstrated for two protein-ligand interactions, an enzyme-small molecule inhibitor complex, and a protein-disaccharide complex. It is shown that the introduction of gas phase imidazole into the ES ion source effectively protects gas phase protein-ligand complexes against in-source dissociation. The stabilizing effect of imidazole vapor is comparable to that observed upon addition of imidazole to the ES solution. The introduction of sulfur hexafluoride, at high partial pressure, into the source region also effectively suppresses in-source dissociation of protein complexes. It is proposed that evaporative cooling is the primary mechanism responsible for the stabilizing effects observed for the gas phase additives.

引用

页码：7801 / 7806

页数：6

共 22 条

[1] Gas-phase proton-transfer chemistry coupled with TOF mass spectrometry and ion mobility-MS for the facile analysis of poly(ethylene glycols) and PEGylated polypeptide conjugates [J].