Spatial organization of the somatosensory cortex revealed by osmFISH

被引：365

作者：

Codeluppi, Simone ^{[1
,2
,3
]}

Borm, Lars E. ^{[1
,3
]}

Zeisel, Amit ^{[1
,3
,5
]}

La Manno, Gioele ^{[1
,3
]}

van Lunteren, Josina A. ^{[4
]}

Svensson, Camilla I. ^{[2
]}

Linnarsson, Sten ^{[1
,3
]}

机构：

[1] Karolinska Inst, Div Mol Neurobiol, Dept Med Biochem & Biophys, Stockholm, Sweden

[2] Karolinska Inst, Dept Physiol & Pharmacol, Stockholm, Sweden

[3] Sci Life Lab, Stockholm, Sweden

[4] Karolinska Inst, Dept Biosci & Nutr, Stockholm, Sweden

[5] Technion, Fac Biotechnol & Food Engn, Haifa, Israel

来源：

NATURE METHODS | 2018年 / 15卷 / 11期

基金：

瑞典研究理事会;

关键词：

RNA; VISUALIZATION; TISSUE;

D O I：

10.1038/s41592-018-0175-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Global efforts to create a molecular census of the brain using single-cell transcriptomics are producing a large catalog of molecularly defined cell types. However, spatial information is lacking and new methods are needed to map a large number of cell type-specific markers simultaneously on large tissue areas. Here, we describe a cyclic single-molecule fluorescence in situ hybridization methodology and define the cellular organization of the somatosensory cortex.

引用

页码：932 / +

页数：6

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