Evidence for a quinone binding site close to the interface between NUOD and NUOB subunits of Complex I

被引：48

作者：

Prieur, I ^{[1
]}

Lunardi, J ^{[1
]}

Dupuis, A ^{[1
]}

机构：

[1] CEA Grenoble, Dept Biol Mol & Struct, Lab Bioenerget Cellulaire & Pathol, UJF,EA 2943, F-38054 Grenoble 9, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2001年 / 1504卷 / 2-3期

关键词：

Complex I; mitochondria; piericidin; rotenone; ubiquinone; Rhodobacter capsulatus;

D O I：

10.1016/S0005-2728(01)00158-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Piericidin, rotenone and pyridaben are specific inhibitors of the NADH-ubiquinone oxidoreductase (Complex I) that bind to its ubiquinone binding site(s). Using site directed mutagenesis, we demonstrate that residues G409, D412, R413 and V407 of the C-terminus of Complex I NUOD subunit are directly involved in the binding of these inhibitors. We propose that the corresponding inhibitor/quinone binding site would be located close to NUOD-NUOB interface. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：173 / 178

页数：6

共 20 条

[1] Function of conserved acidic residues in the PSST homologue of complex I (NADH:Ubiquinone oxidoreductase) from Yarrowia lipolytica [J].

Ahlers, PM ;

Zwicker, K ;

Kerscher, S ;

Brandt, U .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (31) :23577-23582

[2] Proton translocation in the respiratory chain involving ubiquinone - a hypothetical semiquinone switch mechanism for complex I [J].

Brandt, U .

BIOFACTORS, 1999, 9 (2-4) :95-101

[3]

Brandt U, 1998, BBA-BIOENERGETICS, V1364, P85

[4] The 49-kDa subunit of NADH-ubiquinone oxidoreductase (Complex I) is involved in the binding of piericidin and rotenone, two quinone-related inhibitors [J].

Darrouzet, E ;

Issartel, JP ;

Lunardi, J ;

Dupuis, A .

FEBS LETTERS, 1998, 431 (01) :34-38

[5] Distal genes of the nuo operon of Rhodobacter capsulatus equivalent to the mitochondrial ND subunits are all essential for the biogenesis of the respiratory NADH-ubiquinone oxidoreductase [J].

Dupuis, A ;

Darrouzet, E ;

Duborjal, H ;

Pierrard, B ;

Chevallet, M ;

van Belzen, R ;

Albracht, SPJ ;

Lunardi, J .

MOLECULAR MICROBIOLOGY, 1998, 28 (03) :531-541

[6] A reductant-induced oxidation mechanism for Complex I [J].

Dutton, PL ;

Moser, CC ;

Sled, VD ;

Daldal, F ;

Ohnishi, T .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1364 (02) :245-257

[7] PHOTOLABELING OF A MITOCHONDRIALLY ENCODED SUBUNIT OF NADH DEHYDROGENASE WITH [H-3] DIHYDROROTENONE [J].

EARLEY, FGP ;

PATEL, SD ;

RAGAN, CI ;

ATTARDI, G .

FEBS LETTERS, 1987, 219 (01) :108-113

[8] Inhibitors of NADH-ubiquinone reductase: an overview [J].

Esposti, MD .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS, 1998, 1364 (02) :222-235

[9] A motif for quinone binding sites in respiratory and photosynthetic systems [J].

Fisher, N ;

Rich, PR .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 296 (04) :1153-1162

[10] The respiratory complex I of bacteria, archaea and eukarya and its module common with membrane-bound multisubunit hydrogenases [J].

Friedrich, T ;

Scheide, D .

FEBS LETTERS, 2000, 479 (1-2) :1-5

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