A torrid zone on mouse chromosome 1 containing a cluster of recombinational hotspots

被引：28

作者：

Kelmenson, PM ^{[1
]}

Petkov, P ^{[1
]}

Wang, XS ^{[1
]}

Higgins, DC ^{[1
]}

Paigen, BJ ^{[1
]}

Paigen, K ^{[1
]}

机构：

[1] Jackson Lab, Bar Harbor, ME 04609 USA

来源：

GENETICS | 2005年 / 169卷 / 02期

关键词：

D O I：

10.1534/genetics.104.035063

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Within the 2.38-Mb Ath1 region of mouse chromosome 1, 42 of 45 genetic crossovers from crosses between C57BL/6J (B6) and either C3H/HeJ (H) or Mus spretus (SPRET) occurred in four zones (A-D); zone A, 100 kb long, contained a cluster of at least four recombination hotspots. F, sperm assays indicate that within this "torrid zone" the most active hotspot (A3) can initiate recombination on H and SPRET but not B6 chromosomes. The A3 DNA sequence contains a (G/C)TTT repeat, long stretches of A or T, and a cyclic variation in AT content. Recombination was drastically reduced in a cross between B6 and a B6.SPRET Ath1 congenic strain, but was unaffected in a B6 X B6.H Ath1 congenic cross. Similar nonrandom clustering of hotspots has been observed in yeast and the major histocompatibility complexes of human and mouse. To the extent that torrid zones are a general feature of mammalian genomes, they have considerable implications for genetic mapping strategies in both human populations and mouse crosses.

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页码：833 / 841

页数：9

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