A human homolog of the yeast gene encoding tRNA 2′-phosphotransferase:: cloning, characterization and complementation analysis

被引：11

作者：

Hu, QD ^{[1
]}

Lu, H ^{[1
]}

Huo, K ^{[1
]}

Ying, K ^{[1
]}

Li, J ^{[1
]}

Xie, Y ^{[1
]}

Mao, Y ^{[1
]}

Li, YY ^{[1
]}

机构：

[1] Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2003年 / 60卷 / 08期

关键词：

human TRPT1; Saccharomyces cerevisiae TPT1; tRNA splicing; DUF60; domain; Northern blot; functional complementation; plasmid shuffle;

D O I：

10.1007/s00018-003-3107-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Saccharomyces cerevisiae TPT1 gene plays a role in removing the 2'-phosphate from ligated tRNA during the maturation of pre-tRNA. Here we reported the cloning and characterization of the human TRPT1 gene as a homolog of yeast TPT1. The TRPT1 gene is located at human chromosome 11q13 and encodes a polypeptide of 253 amino acids. BLAST searches with its amino acid sequence revealed the ubiquitous occurrence of TRPT1 homologs and their functional relationships with the presence of the DUF60/KptA domain. Northern analysis demonstrated that the gene is primarily expressed in heart and skeletal muscle, with lower or undetectable levels in other tissues studied. A plasmid-shuffling experiment showed that the human TRPT1 gene could complement the tpt1 mutation in S. cerevisiae.

引用

页码：1725 / 1732

页数：8

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