High-throughput scanning with single-molecule sensitivity

被引：5

作者：

Sonnleitner, M ^{[1
]}

Freudenthaler, G ^{[1
]}

Hesse, J ^{[1
]}

Schütz, GJ ^{[1
]}

机构：

[1] Upper Austrian Res GmbH, Ctr Biomed Nanotechnol, A-4020 Linz, Austria

来源：

IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES AND CELLS: FUNDAMENTALS AND APPLICATIONS III | 2005年 / 5699卷

关键词：

single-molecule detection; high-throughput scanning; autofocus; microarray readout; CytoScout;

D O I：

10.1117/12.590292

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We report on the application of a novel fluorescence-microscope based scanning device with single molecule sensitivity to microarray readout. The device is based on a CCD camera operated in time delay and integration (TDI) mode synchronized with the movement of a sample scanning stage, enabling continuous data acquisition. The implementation of a focus hold system keeps the sample in focus during scanning. Results from ultra-sensitive high-resolution microarray readout provide clear evidence for the superiority of the novel detection method over conventional microarray readout systems in particular regarding to sensitivity and dynamic range. Minute sample amount and lacking amplification methods will demand for this ultra-sensitive readout technology in future genomic and proteomic research.

引用

页码：202 / 210

页数：9

共 22 条

[1] Gene-expression profiles predict survival of patients with lung adenocarcinoma [J].

Beer, DG ;

Kardia, SLR ;

Huang, CC ;

Giordano, TJ ;

Levin, AM ;

Misek, DE ;

Lin, L ;

Chen, GA ;

Gharib, TG ;

Thomas, DG ;

Lizyness, ML ;

Kuick, R ;

Hayasaka, S ;

Taylor, JMG ;

Iannettoni, MD ;

Orringer, MB ;

Hanash, S .

NATURE MEDICINE, 2002, 8 (08) :816-824

[2] Image metrics in the statistical analysis of DNA microarray data [J].

Brown, CS ;

Goodwin, PC ;

Sorger, PK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (16) :8944-8949

[3]

Chang K H, 2000, Korean J Radiol, V1, P25

[4] Simultaneous dual-color and dual-polarization imaging of single molecules [J].

Cognet, L ;

Harms, GS ;

Blab, GA ;

Lommerse, PHM ;

Schmidt, T .

APPLIED PHYSICS LETTERS, 2000, 77 (24) :4052-4054

[5] Probing the interaction between two single molecules: Fluorescence resonance energy transfer between a single donor and a single acceptor [J].

Ha, T ;

Enderle, T ;

Ogletree, DF ;

Chemla, DS ;

Selvin, PR ;

Weiss, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (13) :6264-6268

[6] Single-molecule anisotropy imaging [J].

Harms, GS ;

Sonnleitner, M ;

Schütz, GJ ;

Gruber, HJ ;

Schmidt, T .

BIOPHYSICAL JOURNAL, 1999, 77 (05) :2864-2870

[7] Ultra-sensitive fluorescence reader for bioanalysis [J].

Hesse, J ;

Sonnleitner, M ;

Schütz, GJ .

CURRENT PHARMACEUTICAL BIOTECHNOLOGY, 2004, 5 (03) :309-319

[8] Single-molecule reader for high-throughput bioanalysis [J].

Hesse, J ;

Sonnleitner, M ;

Sonnleitner, A ;

Freudenthaler, G ;

Jacak, J ;

Höglinger, O ;

Schindler, H ;

Schütz, GJ .

ANALYTICAL CHEMISTRY, 2004, 76 (19) :5960-5964

[9] Microarray analysis and organization of circadian gene expression Drosophila [J].

McDonald, MJ ;

Rosbach, M .

CELL, 2001, 107 (05) :567-578

[10] Gene expression profiling reveals alterations of specific metabolic pathways in schizophrenia [J].

Middleton, FA ;

Mirnics, K ;

Pierri, JN ;

Lewis, DA ;

Levitt, P .

JOURNAL OF NEUROSCIENCE, 2002, 22 (07) :2718-2729

← 1 2 3 →