On-demand droplet spotter for preparing pico- to femtoliter droplets on surfaces

被引：75

作者：

Yogi, O

Kawakami, T

Yamauchi, M

Ye, JY

Ishikawa, M

机构：

[1] ATP, JRCAT, Tsukuba, Ibaraki 3050046, Japan

[2] Hamamatsu Photon KK Cent Res Lab, Hamakita, Shizuoka 4340041, Japan

来源：

ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 08期

关键词：

D O I：

10.1021/ac0012039

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A droplet spotter for on-demand generation of pico- to femtoliter droplets was developed to meet the requirement for high-density spotting of chemicals on a surface, Our droplet spotter involves applying a similar to 1000-V and similar to 10ms pulse voltage to the tip of a capillary tube (o.d. similar to 18 mum; i.d. similar to 11 pm) supplied with water or a dye solution. The capability of the spotter was demonstrated by preparing a microarray of dye molecules. The microarray was prepared by spotting similar to 30-fL droplets of a dye solution on a surface at the density of one spot per 20 x 20 mum(2).

引用

页码：1896 / 1902

页数：7

共 20 条

[1]

Barnes MD, 1999, CYTOMETRY, V36, P169, DOI 10.1002/(SICI)1097-0320(19990701)36:3<169::AID-CYTO4>3.0.CO

[2]

2-I

[3] DETECTION OF SINGLE RHODAMINE-6G MOLECULES IN LEVITATED MICRODROPLETS [J].