Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns

被引：84

作者：

Burns, C

Gregory, KE

Kirby, M

Cheung, MK

Riquelme, M

Elliott, TJ

Challen, MP

Bailey, A

Foster, GD

机构：

[1] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England

[2] Hort Res Int, Warwick CV35 9EF, England

[3] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England

来源：

FUNGAL GENETICS AND BIOLOGY | 2005年 / 42卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/j.fgb.2004.11.005

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have developed a "Molecular Toolkit" comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes Agaricus bisporus and Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the C. cinereus trp1 promoter and A. bisporus trp2 and gpdII promoters proving successful in driving expression in C. cinereus, with the gpdII promoter also functioning in A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in C. cinereus and A. bisporus is the presence of an intron. This is the first reported expression of GFP in either C. cinereus or A. bisporus. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：191 / 199

页数：9

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