Structural conservation of neurotropism-associated VspA within the variable Borrelia Vsp-OspC lipoprotein family

被引:39
作者
Zücker, WR [1 ]
Kerentseva, TA
Lawson, CL
Barbour, AG
机构
[1] Univ Calif Irvine, Coll Med, Dept Microbiol & Mol Genet, Irvine, CA 92697 USA
[2] Univ Calif Irvine, Coll Med, Dept Med, Irvine, CA 92697 USA
[3] Rutgers State Univ, Dept Chem, Piscataway, NJ 08854 USA
关键词
D O I
10.1074/jbc.M008449200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Vsp surface lipoproteins are serotype-defining antigens of relapsing fever spirochetes that undergo multiphasic antigenic variation to avoid the immune response. One of these proteins, VspA of Borrelia turicatae, is also associated with neurotropism in infected mice. Vsp proteins are highly polymorphic in sequence, which may relate to their specific antibody reactivities and host cell interactions. To determine whether sequence variations affect protein structure, we compared B. turicatae VspA with three related proteins: VspB of B. turicatae, Vsp26 of the relapsing fever agent Borrelia hermsii, and OspC of the Lyme disease spirochete Borrelia burgdorferi. Recombinant mon-lipidated proteins were purified by affinity or ion exchange chromatography. Circular dichroism spectra revealed similar, highly alpha -helical secondary structures for all four proteins. In vitro assays demonstrated protease-resistant, thermostable Vsp cores starting at, a conserved serine at position 34 (Ser(34)). All proteins aggregate as dimers in solution. In situ trypsin treatment and surface protein cross-linking showed that the native lipoproteins also form protease-resistant dimers. These findings indicate that Vsp proteins have a common compact fold and that their established functions are based on localized polymorphisms. Two forms of VspA crystals suitable for structure determination by x-ray diffraction methods have been obtained.
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页码:457 / 463
页数:7
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共 71 条
  • [11] IMMUNOGENIC INTEGRAL MEMBRANE-PROTEINS OF BORRELIA-BURGDORFERI ARE LIPOPROTEINS
    BRANDT, ME
    RILEY, BS
    RADOLF, JD
    NORGARD, MV
    [J]. INFECTION AND IMMUNITY, 1990, 58 (04) : 983 - 991
  • [12] Access of antibody or trypsin to an integral outer membrane protein (P66) of Borrelia burgdorferi is hindered by Osp lipoproteins
    Bunikis, J
    Barbour, AG
    [J]. INFECTION AND IMMUNITY, 1999, 67 (06) : 2874 - 2883
  • [13] A surface-exposed region of a novel outer membrane protein (P66) of Borrelia spp. is variable in size and sequence
    Bunikis, J
    Luke, CJ
    Bunikiene, E
    Bergström, S
    Barbour, AG
    [J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (07) : 1618 - 1623
  • [14] VARIABILITY OF A BACTERIAL SURFACE PROTEIN AND DISEASE EXPRESSION IN A POSSIBLE MOUSE MODEL OF SYSTEMIC LYME BORRELIOSIS
    CADAVID, D
    THOMAS, DD
    CRAWLEY, R
    BARBOUR, AG
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1994, 179 (02) : 631 - 642
  • [15] Immunologic and genetic analyses of VmpA of a neurotropic strain of Borrelia turicatae
    Cadavid, D
    Pennington, PM
    Kerentseva, TA
    Bergstrom, S
    Barbour, AG
    [J]. INFECTION AND IMMUNITY, 1997, 65 (08) : 3352 - 3360
  • [16] EXPERIMENTAL-INFECTION OF THE MOUSE-BRAIN BY A RELAPSING FEVER BORRELIA SPECIES - A MOLECULAR ANALYSIS
    CADAVID, D
    BUNDOC, V
    BARBOUR, AG
    [J]. JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (01) : 143 - 151
  • [17] The properties and function of the glycosylphosphatidylinositol-phospholipase C in Trypanosoma brucei
    Carrington, M
    Carnall, N
    Crow, MS
    Gaud, A
    Redpath, MB
    Wasunna, CL
    Webb, H
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1998, 91 (01) : 153 - 164
  • [18] A bacterial genome in flux:: the twelve linear and nine circular extrachromosomal DNAs in an infectious isolate of the Lyme disease spirochete Borrelia burgdorferi
    Casjens, S
    Palmer, N
    van Vugt, R
    Huang, WM
    Stevenson, B
    Rosa, P
    Lathigra, R
    Sutton, G
    Peterson, J
    Dodson, RJ
    Haft, D
    Hickey, E
    Gwinn, M
    White, O
    Fraser, CM
    [J]. MOLECULAR MICROBIOLOGY, 2000, 35 (03) : 490 - 516
  • [19] Plasmin-coated Borrelia burgdorferi degrades soluble and insoluble components of the mammalian extracellular matrix
    Coleman, JL
    Roemer, EJ
    Benach, JL
    [J]. INFECTION AND IMMUNITY, 1999, 67 (08) : 3929 - 3936
  • [20] Plasminogen is required for efficient dissemination of B-burgdorferi in ticks and for enhancement of spirochetemia in mice
    Coleman, JL
    Gebbia, JA
    Piesman, J
    Degen, JL
    Bugge, TH
    Benach, JL
    [J]. CELL, 1997, 89 (07) : 1111 - 1119