Nuclear lamins A and B1: Different pathways of assembly during nuclear envelope formation in living cells

被引:317
作者
Moir, RD [1 ]
Yoon, M [1 ]
Khuon, S [1 ]
Goldman, RD [1 ]
机构
[1] Northwestern Univ, Sch Med, Dept Cell & Mol Biol, Chicago, IL 60611 USA
关键词
nuclear envelope; mitosis; chromatin; intermediate filaments; green fluorescent protein;
D O I
10.1083/jcb.151.6.1155
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
At the end of mitosis, the nuclear lamins assemble to form the nuclear lamina during nuclear envelope formation in daughter cells. We have fused A- and B-type nuclear lamins to the green fluorescent protein to study this process in living cells. The results reveal that the A- and B-type lamins exhibit different pathways of assembly. In the early stages of mitosis, both lamins are distributed throughout the cytoplasm in a diffusible (nonpolymerized) state, as demonstrated by fluorescence recovery after photobleaching (FRAP). During the anaphase-telophase transition, lamin B1 begins to become concentrated at the surface of the chromosomes. As the chromosomes reach the spindle poles, virtually all of the detectable lamin B1 has accumulated at their surfaces. Subsequently, this lamin rapidly encloses the entire perimeter of the region containing decondensing chromosomes in each daughter cell. By this time, lamin B1 has assembled into a relatively stable polymer, as indicated by FRAP analyses and insolubility in detergent/high ionic strength solutions. In contrast, the association of lamin A with the nucleus begins only after the major components of the nuclear envelope including pore complexes are assembled in daughter cells. Initially lamin A is found in an unpolymerized state throughout the nucleoplasm of daughter cell nuclei in early G1 and only gradually becomes incorporated into the peripheral lamina during the first few hours of this stage of the cell cycle. In later stages of G1, FRAP analyses suggest that both green fluorescent protein lamins A and B1 form higher order polymers throughout interphase nuclei.
引用
收藏
页码:1155 / 1168
页数:14
相关论文
共 78 条
  • [61] DISASSEMBLY OF INVITRO FORMED LAMIN HEAD-TO-TAIL POLYMERS BY CDC2 KINASE
    PETER, M
    HEITLINGER, E
    HANER, M
    AEBI, U
    NIGG, EA
    [J]. EMBO JOURNAL, 1991, 10 (06) : 1535 - 1544
  • [62] INVITRO POSTTRANSLATIONAL MODIFICATION OF LAMIN-B CLONED FROM A HUMAN T-CELL LINE
    POLLARD, KM
    CHAN, EKL
    GRANT, BJ
    SULLIVAN, KF
    TAN, EM
    GLASS, CA
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1990, 10 (05) : 2164 - 2175
  • [63] ROBER RA, 1989, DEVELOPMENT, V105, P365
  • [64] SASSEVILLE AMJ, 1995, J CELL SCI, V108, P273
  • [65] Disruption of nuclear lamin organization alters the distribution of replication factors and inhibits DNA synthesis
    Spann, TP
    Moir, RD
    Goldman, AE
    Stick, R
    Goldman, RD
    [J]. JOURNAL OF CELL BIOLOGY, 1997, 136 (06) : 1201 - 1212
  • [66] TERATOCARCINOMA STEM-CELLS AND EARLY MOUSE EMBRYOS CONTAIN ONLY A SINGLE MAJOR LAMIN POLYPEPTIDE CLOSELY RESEMBLING LAMIN-B
    STEWART, C
    BURKE, B
    [J]. CELL, 1987, 51 (03) : 383 - 392
  • [67] Nuclear lamins: Their structure, assembly, and interactions
    Stuurman, N
    Heins, S
    Aebi, U
    [J]. JOURNAL OF STRUCTURAL BIOLOGY, 1998, 122 (1-2) : 42 - 66
  • [68] Nuclear membrane vesicle targeting to chromatin in a Drosophila embryo cell-free system
    Ulitzur, N
    Harel, A
    Goldberg, M
    Feinstein, N
    Gruenbaum, Y
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1997, 8 (08) : 1439 - 1448
  • [69] LAMIN ACTIVITY IS ESSENTIAL FOR NUCLEAR-ENVELOPE ASSEMBLY IN A DROSOPHILA EMBRYO CELL-FREE-EXTRACT
    ULITZUR, N
    HAREL, A
    FEINSTEIN, N
    GRUENBAUM, Y
    [J]. JOURNAL OF CELL BIOLOGY, 1992, 119 (01) : 17 - 25
  • [70] A DISTINCT VESICLE POPULATION TARGETS MEMBRANES AND PORE COMPLEXES TO THE NUCLEAR-ENVELOPE IN XENOPUS EGGS
    VIGERS, GPA
    LOHKA, MJ
    [J]. JOURNAL OF CELL BIOLOGY, 1991, 112 (04) : 545 - 556