Effect of Surface Charge on the Cellular Uptake and Cytotoxicity of Fluorescent Labeled Cellulose Nanocrystals

被引：254

作者：

Mahmoud, Khaled A. ^{[1
]}

Mena, Jimmy A. ^{[1
]}

Male, Keith B. ^{[1
]}

Hrapovic, Sabahudin ^{[1
]}

Kamen, Amine ^{[1
]}

Luong, John H. T. ^{[1
]}

机构：

[1] Natl Res Council Canada, Biotechnol Res Inst, Montreal, PQ H4P 2R2, Canada

来源：

ACS APPLIED MATERIALS & INTERFACES | 2010年 / 2卷 / 10期

关键词：

cellulose nanocrystal; fluorescence; rhodamine B isothiocyanate; fluorescein isothiocyanate; cytotoxicity; cell imaging; drug delivery; GOLD NANOPARTICLES; SILICA NANOPARTICLES; QUANTUM DOTS; DRUG; PH; CHEMISTRY; DELIVERY; WHISKERS; CELLS;

D O I：

10.1021/am1006222

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Probing of cellular uptake and cytocoxicicy was conducted for two fluorescent cellulose nanocrystals (CNCs): CNC-fluorescein isothiocyanate (FITC) and newly synthesized CNC-rhodamine B isothiocyanate (RBITC). The positively charged CNC-RBITC was uptaken by human embryonic kidney 293 (HEK 293) and Spodoptera frugiperda (Sf9) cells without affecting the cell membrane integrity. The cell viability assay and cell-based impedance spectroscopy revealed no noticeably cytotoxic effect of the CNC-RBITC conjugate. However, no significant internalization of negatively charged CNC-FITC was observed at physiological pH. Indeed, the effector cells were surrounded by CNC-FITC, leading to eventual cell rupture. As the surface charge of CNC played an important role in cellular uptake and cytotoxicity, facile surface functionalization together with observed noncytotoxicity rendered modified CNC as a promising candidate for bioimaging and drug delivery systems.

引用

页码：2924 / 2932

页数：9

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