Ultrasonic standing wave removal of microorganisms from suspension in small batch systems

被引：23

作者：

Limaye, MS ^{[1
]}

Hawkes, JJ ^{[1
]}

Coakley, WT ^{[1
]}

机构：

[1] UNIV WALES COLL CARDIFF,SCH PURE & APPL BIOL,CARDIFF CF1 3TL,S GLAM,WALES

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1996年 / 27卷 / 2-3期

关键词：

cell harvesting; bacterial harvesting; yeast harvesting; ultrasound; ultrasonic standing wave;

D O I：

10.1016/S0167-7012(96)00951-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cells in suspension in an ultrasonic standing wave can be manipulated in both batch and flow systems. The removal of log phase Saccharomyces cerevisiae and Escherichia coli from culture medium in a 5 ml cylindrical tube containing a 3 MHz standing wave with a Bessel shaped pressure profile at right angles to the tube axis is reported here. A chamber and transducer/reflector alignment system was developed to provide a high duality standing wave. Cells in the ultrasonic held were first concentrated in a column of clumps along the axis of the sonication chamber. They were collected by controlled sedimentation using pulsed ultrasound. The cell concentration dependent yeast recovery reached 97% (from 7X10(7) cell . ml(-1)) in a 5.5 min experiment. The yield for the smaller bacteria was 72% at best (from 4X10(9) cells . ml(-1)) in 11 min. The optimum cell concentrations for harvesting of both types of cell were similar when expressed as cell volume per unit volume of suspension. The results provide insight into the requirements of achieving high on-line cell recovery using ultrasound.

引用

页码：211 / 220

页数：10

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