Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be identified by the 'Side Population' marker based on Hoechst 33342 efflux. We show that mouse testicular cells also display a 'Side Population' that express Bcrp1 mRNA, the ABC transporter responsible for Hoechst efflux in hematopoietic cells. Inhibition of Hoechst efflux by specific BCRP1 inhibitor Ko143 show that germinal 'Side Population' phenotype is dependent on BCRP1 activity. Analysis of two well-defined models of altered spermatogenesis (W/W-v mutants and cryptorchid male mice) and RNA expression studies of differentiation markers demonstrate that germinal 'Side Population' contains spermatogonial cells. In addition, alpha(6)-integrin and Stra8 germinal stem cell markers, are expressed in the 'Side Population'. In vivo repopulation. assay clearly establishes that testis 'Side Population' in adult mice is highly enriched in male germ stem cells.
机构:
Division of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
Drabent B.
Bode C.
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Division of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
Bode C.
Miosge N.
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Division of Histology, Department of Anatomy, Georg-August Univ. GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
Miosge N.
Herken R.
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Division of Histology, Department of Anatomy, Georg-August Univ. GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
Herken R.
Doenecke D.
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Division of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
机构:
Division of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
Drabent B.
Bode C.
论文数: 0引用数: 0
h-index: 0
机构:
Division of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
Bode C.
Miosge N.
论文数: 0引用数: 0
h-index: 0
机构:
Division of Histology, Department of Anatomy, Georg-August Univ. GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
Miosge N.
Herken R.
论文数: 0引用数: 0
h-index: 0
机构:
Division of Histology, Department of Anatomy, Georg-August Univ. GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen
Herken R.
Doenecke D.
论文数: 0引用数: 0
h-index: 0
机构:
Division of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 GöttingenDivision of Molecular Biology, Department of Biochemistry, Georg-August Univ. Göttingen, D-37073 Göttingen