'Side Population' cells in adult mouse testis express Bcrp1 gene and are enriched in spermatogonia and germinal stem cells

被引:128
作者
Lassalle, B
Bastos, H
Louis, JP
Riou, L
Testart, J
Dutrillaux, B
Fouchet, P [1 ]
Allemand, I
机构
[1] Univ Paris 07, INSERM, CEA,U566, DSV,DRR,Lab Radiosensibilite Cellules Germinales, Fontenay Aux Roses, France
[2] Univ Paris 07, INSERM, CEA,U566, DSV,DRR,Lab Meiose & Maturat Gamet,U566, Fontenay Aux Roses, France
[3] CNRS, FRE 2358, F-45071 Orleans, France
来源
DEVELOPMENT | 2004年 / 131卷 / 02期
关键词
spermatogenesis; stem cells; BCRP1/ABCG2; transplantation;
D O I
10.1242/dev.00918
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be identified by the 'Side Population' marker based on Hoechst 33342 efflux. We show that mouse testicular cells also display a 'Side Population' that express Bcrp1 mRNA, the ABC transporter responsible for Hoechst efflux in hematopoietic cells. Inhibition of Hoechst efflux by specific BCRP1 inhibitor Ko143 show that germinal 'Side Population' phenotype is dependent on BCRP1 activity. Analysis of two well-defined models of altered spermatogenesis (W/W-v mutants and cryptorchid male mice) and RNA expression studies of differentiation markers demonstrate that germinal 'Side Population' contains spermatogonial cells. In addition, alpha(6)-integrin and Stra8 germinal stem cell markers, are expressed in the 'Side Population'. In vivo repopulation. assay clearly establishes that testis 'Side Population' in adult mice is highly enriched in male germ stem cells.
引用
收藏
页码:479 / 487
页数:9
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