Accumulation of amino-polyvinyl alcohol-coated superparamagnetic iron oxide nanoparticles in bone marrow: implications for local stromal cells

被引：3

作者：

Schulze, Frank ^{[1
,2
]}

Gramoun, Azza ^{[3
,4
,5
]}

Crowe, Lindsey A. ^{[3
,4
]}

Dienelt, Anke ^{[1
,6
]}

Akcan, Tugba ^{[1
]}

Hofmann, Heinrich ^{[7
]}

Vallee, Jean-Paul ^{[3
,4
]}

Duda, Georg N. ^{[1
,6
]}

Ode, Andrea ^{[1
,6
]}

机构：

[1] Charite, Julius Wolff Inst, D-13353 Berlin, Germany

[2] Charite, Berlin Brandenburg Sch Regenerat Therapies, D-13353 Berlin, Germany

[3] Univ Hosp Geneva, Dept Radiol, CH-1205 Geneva, Switzerland

[4] Univ Geneva, CH-1205 Geneva, Switzerland

[5] Cairo Univ, Fac Oral & Dent Med, Dept Oral Biol, Cairo, Egypt

[6] Charite, Berlin Brandenburg Ctr Regenerat Therapies, D-13353 Berlin, Germany

[7] Ecole Polytech Fed Lausanne, Lab Powder Technol, CH-1015 Lausanne, Switzerland

来源：

NANOMEDICINE | 2015年 / 10卷 / 14期

关键词：

bone marrow; bone marrow stromal cells; contrast enhancement; MRI; polyvinyl alcohol; superparamagnetic iron oxide nanoparticles; IN-VITRO; CONTRAST AGENTS; PROTEIN CORONA; ALAMAR BLUE; VIVO; DIFFERENTIATION; PARTICOKINETICS; NANOTOXICOLOGY; DOSIMETRY; TISSUES;

D O I：

10.2217/NNM.15.62

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

First, it will be investigated if amino-polyvinyl alcohol-coated superparamagnetic iron oxide nanoparticles (A-PVA-SPIONs) are suitable for MRI contrast enhancement in bone marrow. Second, the impact of A-PVA-SPION exposure in vivo on the viability and key functions of local bone marrow stromal cells (BMSCs) will be investigated. Material & methods: Animals were systemically injected with A-PVA-SPIONs, followed by a 7-day survival time. Accumulation of A-PVA-SPIONs was confirmed by MRI, histology and inductively coupled plasma optical emission spectrometry. BMSCs were isolated from bone marrow for in vitro assessment of their viability and regenerative key functions. Results: In this study, A-PVA-SPIONs were found to accumulate in bone marrow and increase the BMSCs' metabolic activity and migration rate. Conclusion: A-PVA-SPIONs appear suitable for contrast enhancement in bone marrow while our data suggest an influence on the BMSCs biology that necessitates future research.

引用

页码：2139 / 2151

页数：13

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