Properties, production, and applications of camelid single-domain antibody fragments

被引：594

作者：

Harmsen, M. M.

De Haard, H. J.

机构：

[1] Univ Wageningen & Res Ctr, Inst Anim Sci & Hlth, NL-8219 PH Lelystad, Netherlands

[2] Ablynx NV, B-9052 Ghent, Belgium

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2007年 / 77卷 / 01期

关键词：

single-domain; microbial production; yeast; glycosylation;

D O I：

10.1007/s00253-007-1142-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Camelids produce functional antibodies devoid of light chains of which the single N-terminal domain is fully capable of antigen binding. These single-domain antibody fragments (VHHs or Nanobodies (R)) have several advantages for biotechnological applications. They are well expressed in microorganisms and have a high stability and solubility. Furthermore, they are well suited for construction of larger molecules and selection systems such as phage, yeast, or ribosome display. This minireview offers an overview of (1) their properties as compared to conventional antibodies, (2) their production in microorganisms, with a focus on yeasts, and (3) their therapeutic applications.

引用

收藏

页码：13 / 22

页数：10

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