Cloning and expression of D-lactonohydrolase cDNA from Fusarium moniliforme in Saccharomyces cerevisiae

被引：30

作者：

Liu, ZQ

Sun, ZH ^{[1
]}

机构：

[1] So Yangtze Univ, Sch Biotechnol, Lab Biocatalysis, Wuxi 214036, Peoples R China

[2] Minist Educs, Key Lab Ind Biotechnol, Wuxi 214036, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 24期

基金：

中国国家自然科学基金;

关键词：

cDNA; cloning; D-lactonohydrolase; eukaryotic expression; Fusarium moniliforme;

D O I：

10.1007/s10529-004-5320-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A D-lactonohydrolase gene of about 1.1 kb was cloned from Fusarium moniliforme. The ORF sequence predicted a protein of 382 amino acids with a molecular mass of about 40 kDa. An expression plasmid carrying the gene under the control of the triose phosphate isomerase gene promotor was introduced into Saccharomyces cerevisiae, and the D-lactonohydrolase gene was successfully expressed in the recombinant strains.

引用

页码：1861 / 1865

页数：5

共 16 条

[1]

[Anonymous], METHOD ENZYMOL

[2]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[3] ONE-STEP PREPARATION OF COMPETENT ESCHERICHIA-COLI - TRANSFORMATION AND STORAGE OF BACTERIAL-CELLS IN THE SAME SOLUTION [J].