Attachment of Cryptosporidium parvum sporozoites to human intestinal epithelial cells

被引：42

作者：

Joe, A

Verdon, R

Tzipori, S

Keusch, GT

Ward, HD

机构：

[1] Tufts Univ, Div Geog Med & Infect Dis, New England Med Ctr, Sch Med, Boston, MA 02111 USA

[2] Tufts Univ, Sch Vet Med, Div Infect Dis, N Grafton, MA 01536 USA

来源：

INFECTION AND IMMUNITY | 1998年 / 66卷 / 07期

关键词：

D O I：

10.1128/IAI.66.7.3429-3432.1998

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

An enzyme-linked immunosorbent assay-based attachment model using the human intestinal cell line Caco-2A was developed to study attachment.of Cryptosporidium parvum sporozoites in vitro and to assess potential inhibitors of sporozoite binding. In this system, attachment was related to sporozoite dose, incubation time, and host cell differentiation status. Polyclonal antibodies to C. parvum as well as glycoprotein inhibitor's of a sporozoite lectin reduced attachment. This model will be a valuable tool in elucidating specific molecules and mechanisms involved in sporozoite;host cell attachment.

引用

收藏

页码：3429 / 3432

页数：4

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