The adaptor protein Tks5/Fish is required for podosome formation and function, and for the protease-driven invasion of cancer cells

被引：292

作者：

Seals, DF ^{[1
]}

Azucena, EF ^{[1
]}

Pass, I ^{[1
]}

Tesfay, L ^{[1
]}

Gordon, R ^{[1
]}

Woodrow, M ^{[1
]}

Resau, JH ^{[1
]}

Courtneidge, SA ^{[1
]}

机构：

[1] Van Andel Res Inst, Grand Rapids, MI 49503 USA

来源：

CANCER CELL | 2005年 / 7卷 / 02期

关键词：

D O I：

10.1016/j.ccr.2005.01.006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Tks5/Fish is a scaffolding protein with five SH3 domains and one PX domain. In Src-transformed cells, Tks5/Fish localizes to podosomes, discrete protrusions of the ventral membrane. We generated Src-transformed cells with reduced Tks5/Fish levels. They no longer formed podosomes, did not degrade gelatin, and were poorly invasive. We detected Tks5/Fish expression in podosomes in invasive cancer cells, as well as in human breast cancer and melanoma samples. Tks5/Fish expression was also required for protease-driven matrigel invasion in human cancer cells. Finally, coexpression of Tks5/Fish and Src in epithelial cells resulted in the appearance of podosomes. Thus, Tks5/Fish appears to be required for podosome formation, for degradation of the extracellular matrix, and for invasion of some cancer cells.

引用

页码：155 / 165

页数：11

共 39 条

[1] The adaptor protein fish associates with members of the ADAMs family and localizes to podosomes of Src-transformed cells [J].