Changes in endogenous cytokinin profiles in micropropagated Harpagophytum procumbens in relation to shoot-tip necrosis and cytokinin treatments

被引:19
作者
Bairu, Michael W. [1 ]
Novak, Ondrej [2 ,3 ]
Dolezal, Karel [2 ,3 ]
Van Staden, Johannes [1 ]
机构
[1] Univ KwaZulu Natal Pietermaritzburg, Sch Biol & Conservat Sci, Res Ctr Plant Growth & Dev, ZA-3209 Scottsville, South Africa
[2] Palacky Univ, Lab Growth Regulators, Olomouc 78371, Czech Republic
[3] Inst Expt Bot AS CR, Olomouc 78371, Czech Republic
基金
新加坡国家研究基金会;
关键词
Auxin cytokinin interaction; Cytokinin purification and analysis; Devil's claw; Glucosylation; Harpagophytum procumbens; Micropropagation; Pedaliaceae; Shoot-tip necrosis; IN-VITRO; ALOE-POLYPHYLLA; META-TOPOLIN; BENZYLADENINE; GROWTH; PLANTS; BIOSYNTHESIS; SPP;
D O I
10.1007/s10725-010-9558-6
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Changes in cytokinin (CK) profiles and their physiological implications in micropropagated Harpagophytum procumbens [(Burch.) DC. ex Meisn.] tissues in relation to shoot-tip necrosis (STN) and CK treatments were studied. Total CK content was quantified in benzyladenine (BA)-treated necrotic and normal plantlets and in plantlets treated with the CKs BA, meta-topolin (mT) and meta-topolin riboside (mTR) with and without the auxin indole-3-acetic acid (IAA). Generally necrotic shoots yielded more total CK compared to normal shoots. Cytokinin accumulation was higher at the basal section (basal > middle > top). Further analysis of the CKs based on structural and functional forms revealed excessive accumulation of 9-glucosides (deactivation products-toxic metabolites) and limited amounts of O-glucosides (storage forms-re-utilizable) in necrotic and BA-treated shoots compared to normal and topolin-treated cultures. The addition of IAA enhanced the formation of 9-glucosides in BA-treated cultures but reduced it in topolin-treated cultures. The symptom of STN could therefore be attributed to conversion of active cytokinins to other forms such as 9-glucosides which are neither active nor reversibly sequestrated to active forms. Literature shows that metabolites like 9-glucosides of BA have a detrimental effect in plant tissue culture.
引用
收藏
页码:105 / 114
页数:10
相关论文
共 28 条
[21]  
SKOOG F, 1957, Symp Soc Exp Biol, V11, P118
[22]   The commercial harvest of devil's claw (Harpagophytum spp.) in southern Africa:: The devil's in the details [J].
Stewart, KM ;
Cole, D .
JOURNAL OF ETHNOPHARMACOLOGY, 2005, 100 (03) :225-236
[23]   The elusive cytokinin biosynthetic pathway [J].
Taylor, NJ ;
Stirk, WA ;
van Staden, J .
SOUTH AFRICAN JOURNAL OF BOTANY, 2003, 69 (03) :269-281
[24]   Microcutting leaf area, weight and position on the stock shoot influence root vigour, shoot growth and incidence of shoot tip necrosis in grape plantlets in vitro [J].
Thomas, P .
PLANT CELL TISSUE AND ORGAN CULTURE, 2000, 61 (03) :189-198
[25]   Osmotic pretreatment promotes axillary shooting from cauliflower curd pieces by acting through internal cytokinin level modifications [J].
Vandemoortele, JL ;
Kevers, C ;
Billard, JP ;
Gaspar, T .
JOURNAL OF PLANT PHYSIOLOGY, 2001, 158 (02) :221-225
[26]  
VIEITEZ AM, 1989, SCI HORTIC, V41, P101, DOI DOI 10.1016/0304-4238(89)90059-9
[27]   THE METABOLISM OF BENZYLADENINE IN SPATHIPHYLLUM-FLORIBUNDUM SCHOTT-PETITE IN RELATION TO ACCLIMATIZATION PROBLEMS [J].
WERBROUCK, SPO ;
VANDERJEUGT, B ;
DEWITTE, W ;
PRINSEN, E ;
VANONCKELEN, HA ;
DEBERGH, PC .
PLANT CELL REPORTS, 1995, 14 (10) :662-665
[28]   Meta-topolin, an alternative to benzyladenine in tissue culture? [J].
Werbrouck, SPO ;
Strnad, M ;
VanOnckelen, HA ;
Debergh, PC .
PHYSIOLOGIA PLANTARUM, 1996, 98 (02) :291-297