Analysis of sperm concentration and motility in a microfluidic device

被引：49

作者：

Chen, Yu-An ^{[1
]}

Huang, Zi-Wei ^{[1
]}

Tsai, Fang-Sheng ^{[2
]}

Chen, Chang-Yu ^{[1
]}

Lin, Cheng-Ming ^{[1
]}

Wo, Andrew M. ^{[1
]}

机构：

[1] Natl Taiwan Univ, Inst Appl Mech, Taipei 106, Taiwan

[2] Natl Taiwan Univ Hosp, Dept Urol, Taipei, Taiwan

来源：

MICROFLUIDICS AND NANOFLUIDICS | 2011年 / 10卷 / 01期

关键词：

Microfluidics; Sperm; Infertility; Resistive pulse technique; MICROFABRICATED DEVICE; SEPARATION; CULTURE; BIOLOGY; SYSTEM;

D O I：

10.1007/s10404-010-0646-8

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A home-use device that allows rapid and quantitative sperm quality analysis is desirable but not yet fully realized. To aid this effort, this article presents a microfluidic device capable of quantifying sperm quality in terms of two critical fertility-related parameters-motile sperm concentration and motility. The microdevice produces flow field for sperms to swim against, and sperms that overcame the flow within a specified time are propelled along in a separate channel and counted via resistive pulse technique. Data are compared to two control methods clinically utilized for sperm quality exam-hemocytometer and the sperm quality analyzer. Results reveal the numbers of pulses generated by passage of sperms correlates strongly with the two control methods: pulse number from 0 to 335 corresponds to progressively motile sperm concentrations from 0 to 19 x 10(6)/ml (hemocytometer) and Sperm Motility Index from 0 to 204 (sperm quality analyzer). The microdevice should be applicable to facilitate self-assessment of sperm quality at home.

引用

页码：59 / 67

页数：9

共 29 条

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