Increased pfmdr1 Copy Number and Sequence Polymorphisms in Plasmodium falciparum Isolates from Sudanese Malaria Patients Treated with Artemether-Lumefantrine

被引：62

作者：

Gadalla, Nahla B. ^{[1
,2
]}

Adam, Ishag ^{[3
]}

Elzaki, Salah-Eldin ^{[2
]}

Bashir, Sahar ^{[4
]}

Mukhtar, Izdihar ^{[4
]}

Oguike, Mary ^{[1
]}

Gadalla, Amal ^{[2
]}

Mansour, Fathi ^{[2
]}

Warhurst, David ^{[5
]}

El-Sayed, Badria B. ^{[2
]}

Sutherland, Colin J. ^{[1
,6
]}

机构：

[1] London Sch Hyg & Trop Med, Fac Infect & Trop Dis, Dept Immunol & Infect, London WC1E 7HT, England

[2] Natl Res Ctr, Dept Epidemiol, Res Inst Trop Med, Khartoum, Sudan

[3] Univ Khartoum, Fac Med, Khartoum, Sudan

[4] Fed Minist Hlth, Natl Hlth Labs, Khartoum, Sudan

[5] London Sch Hyg & Trop Med, Fac Infect & Trop Dis, Dept Pathogen Mol Biol, London WC1E 7HT, England

[6] Hosp Trop Dis, Dept Clin Parasitol, London NW1 0PE, England

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2011年 / 55卷 / 11期

关键词：

ARTEMISININ RESISTANCE; INCREASED SENSITIVITY; DRUG-RESISTANCE; POINT MUTATIONS; GENE; MEFLOQUINE; AMPLIFICATION; ALLELES; SERCA; ANTIMALARIAL;

D O I：

10.1128/AAC.05102-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Molecular markers for surveillance of Plasmodium falciparum resistance to current antimalarials are sorely needed. A 28-day efficacy study of artemether-lumefantrine in eastern Sudan identified 5 treatment failures among 100 evaluable patients; 9 further individuals were parasite positive by PCR during follow-up. Polymorphisms in pfatpase6 and pfmdr1 were evaluated by DNA sequencing. One individual carried parasites with a novel pfmdr1 polymorphism (F1044L). pfmdr1 gene amplification in parasites prior to treatment occurred in three individuals who had recurrent infection during follow-up.

引用

页码：5408 / 5411

页数：4

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