Transcriptome analysis of Pseudomonas aeruginosa growth:: Comparison of gene expression in planktonic cultures and developing and mature biofilms

被引:150
作者
Waite, RD
Papakonstantinopoulou, A
Littler, E
Curtis, MA
机构
[1] Barts & London Queen Marys Sch Med & Dent, MRC, Mol Pathogenesis Res Unit, Ctr Infect Dis,Inst Cell & Mol Sci, London E1 2AT, England
[2] Medivir UK Ltd, Cambridge CB1 9PT, England
关键词
D O I
10.1128/JB.187.18.6571-6576.2005
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The transcriptomes of logarithmic- and stationary-phase Pseudomonas aeruginosa planktonic cultures and static biofilms of different stages of development were compared. Developing and confluent biofilm transcriptomes were found to be related to those of logarithmic- and stationary-phase planktonic cultures, respectively. In addition, a number of novel genes were up-regulated in developing and confluent biofilms, including genes encoding putative solute transport proteins and transcriptional regulators, respectively.
引用
收藏
页码:6571 / 6576
页数:6
相关论文
共 29 条
[1]   Cell cycle-dependent degradation of a flagellar motor component requires a novel-type response regulator [J].
Aldridge, P ;
Jenal, U .
MOLECULAR MICROBIOLOGY, 1999, 32 (02) :379-391
[2]   Global impact of mature biofilm lifestyle on Escherichia coli K-12 gene expression [J].
Beloin, C ;
Valle, J ;
Latour-Lambert, P ;
Faure, P ;
Kzreminski, M ;
Balestrino, D ;
Haagensen, JAJ ;
Molin, S ;
Prensier, G ;
Arbeille, B ;
Ghigo, JM .
MOLECULAR MICROBIOLOGY, 2004, 51 (03) :659-674
[3]   The MerR family of transcriptional regulators [J].
Brown, NL ;
Stoyanov, JV ;
Kidd, SP ;
Hobman, JL .
FEMS MICROBIOLOGY REVIEWS, 2003, 27 (2-3) :145-163
[4]   PSEUDOMONAS-AERUGINOSA INFECTION IN CYSTIC-FIBROSIS - NUCLEOTIDE-SEQUENCE AND TRANSCRIPTIONAL REGULATION OF THE ALGD GENE [J].
DERETIC, V ;
GILL, JF ;
CHAKRABARTY, AM .
NUCLEIC ACIDS RESEARCH, 1987, 15 (11) :4567-4581
[5]   Antimicrobial resistance of Pseudomonas aeruginosa biofilms [J].
Drenkard, E .
MICROBES AND INFECTION, 2003, 5 (13) :1213-1219
[6]   Regulation of gene expression by cell-to-cell communication: Acyl-homoserine lactone quorum sensing [J].
Fuqua, C ;
Parsek, MR ;
Greenberg, EP .
ANNUAL REVIEW OF GENETICS, 2001, 35 :439-468
[7]   GENETIC RECOMBINATION IN PSEUDOMONAS-AERUGINOSA [J].
HOLLOWAY, BW .
JOURNAL OF GENERAL MICROBIOLOGY, 1955, 13 (03) :572-581
[8]   Posttranscriptional control of quorum-sensing-dependent virulence genes by DksA in Pseudomonas aeruginosa [J].
Jude, F ;
Köhler, T ;
Branny, P ;
Perron, K ;
Mayer, MP ;
Comte, R ;
van Delden, C .
JOURNAL OF BACTERIOLOGY, 2003, 185 (12) :3558-3566
[9]   MECHANISMS INVOLVED IN THE EVASION OF THE HOST DEFENSE BY PSEUDOMONAS-AERUGINOSA [J].
KHARAZMI, A .
IMMUNOLOGY LETTERS, 1991, 30 (02) :201-206
[10]   Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants [J].
Klausen, M ;
Heydorn, A ;
Ragas, P ;
Lambertsen, L ;
Aaes-Jorgensen, A ;
Molin, S ;
Tolker-Nielsen, T .
MOLECULAR MICROBIOLOGY, 2003, 48 (06) :1511-1524