Sensitivity by combination: immuno-PCR and related technologies

被引:93
作者
Adler, Michael [1 ]
Wacker, Ron [1 ]
Niemeyer, Christof M. [2 ,3 ]
机构
[1] Chimera Biotec GmbH, D-44227 Dortmund, Germany
[2] Tech Univ Dortmund, Fachbereich Chem, Lehrstuhl Biol Chem Mikrostrukturtech, D-44227 Dortmund, Germany
[3] ISAS Inst Sci Anal, D-44139 Dortmund, Germany
关键词
D O I
10.1039/b718587c
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The versatility of immunoassays for the detection of antigens can be combined with the signal amplification power of nucleic acid amplification techniques in a broad range of innovative detection strategies. This review summarizes the spectrum of both, DNA-modification techniques used for assay enhancement and the resulting key applications. In particular, it focuses on the highly sensitive immuno-PCR (IPCR) method. This technique is based on chimeric conjugates of specific antibodies and nucleic acid molecules, the latter of which are used as markers to be amplified by PCR or related techniques for signal generation and read-out. Various strategies for the combination of antigen detection and nucleic acid amplification are discussed with regard to their laboratory analytic performance, including novel approaches to the conjugation of antibodies with DNA, and alternative pathways for signal amplification and detection. A critical assessment of advantages and drawbacks of these methods for a number of applications in clinical diagnostics and research is conducted. The examples include the detection of viral and bacterial antigens, tumor markers, toxins, pathogens, cytokines and other targets in different biological sample materials.
引用
收藏
页码:702 / 718
页数:17
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