RNA expression analysis from formalin fixed paraffin embedded tissues

被引：147

作者：

Farragher, Susan M. ^{[2
]}

Tanney, Austin ^{[2
]}

Kennedy, Richard D. ^{[2
,3
]}

Harkin, D. Paul ^{[1
,2
]}

机构：

[1] Queens Univ Belfast, Ctr Canc Res & Cell Biol, Belfast BT9 7BL, Antrim, North Ireland

[2] Almac Diagnost, Craigavon, Armagh BT63 5QD, North Ireland

[3] Belfast City Hosp Trust, Belfast BT9 7AB, Antrim, North Ireland

来源：

HISTOCHEMISTRY AND CELL BIOLOGY | 2008年 / 130卷 / 03期

关键词：

FFPE; RNA profiling; microarray; gene expression; personalized medicine;

D O I：

10.1007/s00418-008-0479-7

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Formalin fixation and paraffin embedding (FFPE) is the most commonly used method worldwide for tissue storage. This method preserves the tissue integrity but causes extensive damage to nucleic acids stored within the tissue. As methods for measuring gene expression such as RT-PCR and microarray are adopted into clinical practice there is an increasing necessity to access the wealth of information locked in the Formalin fixation and paraffin embedding archives. This paper reviews the progress in this field and discusses the unique opportunities that exist for the application of these techniques in the development of personalized medicine.

引用

页码：435 / 445

页数：11

共 61 条

[1] Towards quantitative mRNA analysis in paraffin-embedded tissues using real-time reverse transcriptase-polymerase chain reaction - A methodological study on lymph nodes from melanoma patients [J].

Abrahamsen, HN ;

Steiniche, T ;

Nexo, E ;

Hamilton-Dutoit, SJ ;

Sorensen, BS .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2003, 5 (01) :34-41

[2] GENETIC AND CYTOGENETICAL EFFECTS OF FORMALDEHYDE AND RELATED COMPOUNDS [J].

AUERBACH, C ;

MOUTSCHENDAHMEN, M ;

MOUTSCHEN, J .

MUTATION RESEARCH, 1977, 39 (3-4) :317-362

[3] Classification of human lung carcinomas by mRNA expression profiling reveals distinct adenocarcinoma subclasses [J].

Bhattacharjee, A ;

Richards, WG ;

Staunton, J ;

Li, C ;

Monti, S ;

Vasa, P ;

Ladd, C ;

Beheshti, J ;

Bueno, R ;

Gillette, M ;

Loda, M ;

Weber, G ;

Mark, EJ ;

Lander, ES ;

Wong, W ;

Johnson, BE ;

Golub, TR ;

Sugarbaker, DJ ;

Meyerson, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (24) :13790-13795

[4] Quantitative gene expression profiling in formalin-fixed, paraffin-embedded tissues using universal bead arrays [J].

Bibikova, M ;

Talantov, D ;

Chudin, E ;

Yeakley, JM ;

Chen, J ;

Doucet, D ;

Wickham, E ;

Atkins, D ;

Barker, D ;

Chee, M ;

Wang, YX ;

Fan, JB .

AMERICAN JOURNAL OF PATHOLOGY, 2004, 165 (05) :1799-1807

[5] Expression signatures that correlated with Gleason score and relapse in prostate cancer [J].

Bibikova, Marina ;

Chudin, Eugene ;

Arsanjani, Amir ;

Zhou, Lixin ;

Garcia, Eliza Wickham ;

Modder, Joshua ;

Kostelec, Monica ;

Barker, David ;

Downs, Tracy ;

Fan, Jian-Bing ;

Wang-Rodriguez, Jessica .

GENOMICS, 2007, 89 (06) :666-672

[6] THE DURATION OF FIXATION INFLUENCES THE YIELD OF HCV CDNA-PCR PRODUCTS FROM FORMALIN-FIXED, PARAFFIN-EMBEDDED LIVER-TISSUE [J].

BRESTERS, D ;

SCHIPPER, MEI ;

REESINK, HW ;

BOESERNUNNINK, BDM ;

CUYPERS, HTM .

JOURNAL OF VIROLOGICAL METHODS, 1994, 48 (2-3) :267-272

[7] Validation and clinical utility of a 70-gene prognostic signature for women with node-negative breast cancer [J].

Buyse, Marc ;

Loi, Sherene ;

van't Veer, Laura ;

Viale, Giuseppe ;

Delorenzi, Mauro ;

Glas, Annuska M. ;

d'Assignies, Mahasti Saghatchian ;

Bergh, Jonas ;

Lidereau, Rosette ;

Ellis, Paul ;

Harris, Adrian ;

Bogaerts, Jan ;

Therasse, Patrick ;

Floore, Arno ;

Amakrane, Mohamed ;

Piette, Fanny ;

Rutgers, Emiel ;

Sotiriou, Christos ;

Cardoso, Fatima ;

Piccart, Martine J. .

JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE, 2006, 98 (17) :1183-1192

[8] Clinical application of the 70-gene profile: The MINDACT trial [J].

Cardoso, Fatima ;

Van't Veer, Laura ;

Rutgers, Emiel ;

Loi, Sherene ;

Mook, Stella ;

Piccart-Gebhart, Martine J. .

JOURNAL OF CLINICAL ONCOLOGY, 2008, 26 (05) :729-735

[9] Successful application of microarray technology to microdissected formalin-fixed, paraffin-embedded tissue [J].

Coudry, Renata A. ;

Meireles, Sibele I. ;

Stoyanova, Radka ;

Cooper, Harry S. ;

Carpino, Alan ;

Wang, Xianqun ;

Engstrom, Paul F. ;

Clapper, Margie L. .

JOURNAL OF MOLECULAR DIAGNOSTICS, 2007, 9 (01) :70-79

[10] Measurement of gene expression in archival paraffin-embedded tissues - Development and performance of a 92-gene reverse transcriptase-polymerase chain reaction assay [J].

Cronin, M ;

Pho, M ;

Dutta, D ;

Stephans, JC ;

Shak, S ;

Kiefer, MC ;

Esteban, JM ;

Baker, JB .

AMERICAN JOURNAL OF PATHOLOGY, 2004, 164 (01) :35-42

← 1 2 3 4 5 6 7 →